LernerLab
@lernerlab.bsky.social
Account content: Science from laboratory of Eitan Lerner, Hebrew University.
Interests: Biological Fluorescence, Photosynthesis, Single-particle spectroscopy, Dynamic Structural Biology, Super-resolution microscopy... (Life is short yet interesting)
Interests: Biological Fluorescence, Photosynthesis, Single-particle spectroscopy, Dynamic Structural Biology, Super-resolution microscopy... (Life is short yet interesting)
In other words, be careful when using PEG to induce protein phase separation. It will do so not only for proteins with intrinsically disordered proteins but also for well-folded stable ones, with minimal disorder.
March 1, 2025 at 12:01 AM
In other words, be careful when using PEG to induce protein phase separation. It will do so not only for proteins with intrinsically disordered proteins but also for well-folded stable ones, with minimal disorder.
Importantly, it is not only about the bulkiness of a crowder. We compared the effects of PEG & PVA, with similar "softness" and different chemical groups.
Only PEG induced fluorescence lifetime modulation.
PEG's interactions with exposed lysines are unique.
Only PEG induced fluorescence lifetime modulation.
PEG's interactions with exposed lysines are unique.
February 28, 2025 at 11:57 PM
Importantly, it is not only about the bulkiness of a crowder. We compared the effects of PEG & PVA, with similar "softness" and different chemical groups.
Only PEG induced fluorescence lifetime modulation.
PEG's interactions with exposed lysines are unique.
Only PEG induced fluorescence lifetime modulation.
PEG's interactions with exposed lysines are unique.
which is now published in doi.org/10.1002/pro....
PEG–mCherry interactions beyond classical macromolecular crowding
The dense cellular environment influences bio-macromolecular structure, dynamics, interactions, and function. Despite advancements in understanding protein–crowder interactions, predicting their prec...
doi.org
February 19, 2025 at 2:44 PM
which is now published in doi.org/10.1002/pro....
Sure thing. See you soon.
February 11, 2025 at 8:36 PM
Sure thing. See you soon.
As well as a talk in the "Spectroscopy: from forces to photons I" platform, Sunday 17:55.
Come over
Come over
February 11, 2025 at 8:35 PM
As well as a talk in the "Spectroscopy: from forces to photons I" platform, Sunday 17:55.
Come over
Come over
9) As a final remark, I am looking forward to meeting you, existing friends and colleagues, and new potential ones to discuss Science.
February 11, 2025 at 7:39 PM
9) As a final remark, I am looking forward to meeting you, existing friends and colleagues, and new potential ones to discuss Science.
8) Finally, I am taking part in a multi-group study, led by the @timcraggs.bsky.social Lab, which will be presented Wednesday 10:30, poster B478, continuing the exploration of single-molecule fluorescence-based tools for probing distances shorter than ones possible with smFRET.
February 11, 2025 at 7:39 PM
8) Finally, I am taking part in a multi-group study, led by the @timcraggs.bsky.social Lab, which will be presented Wednesday 10:30, poster B478, continuing the exploration of single-molecule fluorescence-based tools for probing distances shorter than ones possible with smFRET.
7) The talk will be given Sunday 17:55 at the "Spectroscopy: from forces to photons I" platform.
Importantly, this work is a close collaboration with the Nir Keren lab, and already feeds exciting follow-up projects (to be continued).
Importantly, this work is a close collaboration with the Nir Keren lab, and already feeds exciting follow-up projects (to be continued).
February 11, 2025 at 7:39 PM
7) The talk will be given Sunday 17:55 at the "Spectroscopy: from forces to photons I" platform.
Importantly, this work is a close collaboration with the Nir Keren lab, and already feeds exciting follow-up projects (to be continued).
Importantly, this work is a close collaboration with the Nir Keren lab, and already feeds exciting follow-up projects (to be continued).
6) The talented postdoc, Paul Harris, will give a talk about his work regarding the multiparameter time-resolved fluorescence-based measurements of photosynthesis-related features in phytoplankton cells one at a time (see doi.org/10.1111/febs...).
FEBS Press
Phytoplankton cells of the same species in the same environment exhibit cell-wide coordination into distinct subpopulations of photophysiological cell states. Upon a rapid change in light intensity, ...
doi.org
February 11, 2025 at 7:39 PM
6) The talented postdoc, Paul Harris, will give a talk about his work regarding the multiparameter time-resolved fluorescence-based measurements of photosynthesis-related features in phytoplankton cells one at a time (see doi.org/10.1111/febs...).
5) As for posters, the research associate, Yair Razvag, will present one about FRETsael (doi.org/10.1101/2023...), which allows localizing FRET pairs of interacting proteins with 20-30 nm precision, using confocal microscopy and non-blinking dyes (Monday 13:45, poster B567).
FRET-sensitized acceptor emission localization (FRETsael) – nanometer localization of biomolecular interactions using fluorescence lifetime imaging
Super-resolution light microscopy techniques facilitate the observation of nanometer-size biomolecules, which are 1-2 orders of magnitude smaller than the diffraction limit of light. Using super-resol...
doi.org
February 11, 2025 at 7:39 PM
5) As for posters, the research associate, Yair Razvag, will present one about FRETsael (doi.org/10.1101/2023...), which allows localizing FRET pairs of interacting proteins with 20-30 nm precision, using confocal microscopy and non-blinking dyes (Monday 13:45, poster B567).
4) Come over on Monday 13:45 to see the poster of
the @sarahrauscher.bsky.social lab, summarizing this work (poster B4).
the @sarahrauscher.bsky.social lab, summarizing this work (poster B4).
February 11, 2025 at 7:39 PM
4) Come over on Monday 13:45 to see the poster of
the @sarahrauscher.bsky.social lab, summarizing this work (poster B4).
the @sarahrauscher.bsky.social lab, summarizing this work (poster B4).
3) This is a collaborative project with the @meshorer.bsky.social lab, and the @sarahrauscher.bsky.social lab.
Talking about @sarahrauscher.bsky.social, they have presented an elaborate MD-based model of the effects of PEG on the FP mCherry, a work we took part in (see doi.org/10.1101/2024...).
Talking about @sarahrauscher.bsky.social, they have presented an elaborate MD-based model of the effects of PEG on the FP mCherry, a work we took part in (see doi.org/10.1101/2024...).
PEG-mCherry interactions beyond classical macromolecular crowding
The dense cellular environment influences bio-macromolecular structure, dynamics, interactions and function. Despite advancements in understanding protein-crowder interactions, predicting their precis...
doi.org
February 11, 2025 at 7:39 PM
3) This is a collaborative project with the @meshorer.bsky.social lab, and the @sarahrauscher.bsky.social lab.
Talking about @sarahrauscher.bsky.social, they have presented an elaborate MD-based model of the effects of PEG on the FP mCherry, a work we took part in (see doi.org/10.1101/2024...).
Talking about @sarahrauscher.bsky.social, they have presented an elaborate MD-based model of the effects of PEG on the FP mCherry, a work we took part in (see doi.org/10.1101/2024...).
2) about how to use FLIM of (monomeric red) fluorescent proteins to probe local densities and their heterogeneities within heterochromatin bio-condensates in mouse embryonic stem cells (see doi.org/10.1038/s414...).
February 11, 2025 at 7:39 PM
2) about how to use FLIM of (monomeric red) fluorescent proteins to probe local densities and their heterogeneities within heterochromatin bio-condensates in mouse embryonic stem cells (see doi.org/10.1038/s414...).