We identified PBX3 as an example of a TF that is closely linked to the inhibitory neuron lineage priming. In silico perturbation experiments of multimodal GRNs suggest PBX3 as a potential mediator of the effect. 🧬🔑

🧵4/6

Our findings show that chronic GC exposure alters lineage specification, with a selective priming of the inhibitory neuron lineage. 🧠

🧵3/6

🚨🧠 New paper alert: Stress alters neuronal balance in the developing brain 🧠🚨

Our latest study in @science.orgAdvances explores how glucocorticoid exposure—a key environmental risk factor—shapes early human brain development using #organoids […]

[Original post on mastodon.social]

Thanks to our amazing PIs @graycamplab.bsky.social , @fabiantheis.bsky.social, and Barbara Treutlein for all their guidance and support! 🚀
This work is part of the larger Human Cell Atlas effort to map all human cells. Explore more @natureportfolio.bsky […]

[Original post on mastodon.social]

Disease Modeling 😷 : Our atlas can be leveraged as a diverse control cohort to contextualise organoid models of disease, helping identify underlying genes and pathways. We found that comprehensive control data is vital for disentangling disease from regional effects.

🧵5/8

Quantifying Organoid Fidelity 📊: By estimating the transcriptomic similarity between primary and organoid brain counterparts, we provide a robust framework for assessing protocol variation and fidelity across labs and methods. Cell stress seems to be quite dependent on protocol.

🧵4/8

Identifying Missing Cell States 🔍: We map neural organoid cell types and states to a developing human brain reference, highlighting gaps in the diversity of brain regions present in current in vitro models. ⚠️While the telencephalon seems well-captured […]

[Original post on mastodon.social]

Introducing the Human Neural Organoid Cell Atlas 🗺️: We’ve integrated 36 neural organoid datasets across 26 protocols. This produced a comprehensive, integrated reference atlas, including a hierarchical cell-type annotation, partially derived by mapping to primary human 🧠.

🧵2/8