We hope this will open up further applications for fast 3D imaging of tissues and complex structures with sub-cellular resolution, using conventional sample mounting techniques.
Thank you to everyone who made this work possible.
Full details 👉 doi.org/10.1364/BOE.... (🧵 6/6)

We demonstrate our method on biological samples with a range of RIs, including a whole fly brain 🧠 (imaged in < 3 min⚡) and > 1 cm long mouse bone 🐭🦴 (🧵 5/6).

Using RI-tuned bead phantoms, we show that correction collar adjustment can partially compensate for RI mismatch, improving the imaging depth for a range of clearing protocols 🧪 (🧵 4/6).

We demonstrate imaging of higher refractive indices (RI), maintaining a sub-1.5 µm axial resolution over hundreds of microns in depth, with multi-view image fusion providing more isotropic resolution (🧵 3/6).

We present cleared-tissue optimized single-objective dual-view light-sheet fluorescence microscopy using:
🔹Folded remote-refocusing OPM
🔹1.25 NA silicone-immersion primary objective
(🧵2/6).

We hope this will open up further applications for sub-cellular resolution 3D imaging of tissues and complex structures, using conventional sample preparation and mounting techniques 🔬.
Thank you to everyone who made this work possible.
Full details 👉 doi.org/10.1364/BOE.... (🧵6/6)

We demonstrate our method on biological samples with a range of RIs, including a whole fly brain 🧠 (imaged in < 3 min⚡) and >1 cm-long mouse bone 🐭🦴 (🧵5/6).

Using RI-tuned bead phantoms, we show that correction collar adjustment can partially compensate for RI mismatch, improving the imaging depth for a range of clearing protocols 🧪 (🧵4/6).

We demonstrate imaging of higher refractive indices (RI), maintaining a sub-1.5 µm axial resolution over hundreds of microns in depth, with multi-view image fusion providing more isotropic resolution (🧵3/6).

We present cleared-tissue optimized single-objective dual-view light-sheet fluorescence microscopy with a folded remote-refocusing OPM system configured with a 1.25 NA silicone-immersion primary objective (🧵2/6).