Puhar Lab
@lab-puhar.bsky.social
We study gut bacteria and the immune response to infection with a focus on the danger signal extracellular ATP. We particularly like Shigella and E. coli. Located at Queen's University Belfast, UK and Umeå University, Sweden.
💰We are very thankful to @kawresearch.bsky.social, @vetenskapsradet.bsky.social, SSFM, Kempe Stiftelserna, @mimsumea.bsky.social, @umeauniversitet.bsky.social, @qubelfastofficial.bsky.social, @wwiem-qub.bsky.social for support and helpful staff at @cp-cellreports.bsky.social!
October 14, 2025 at 5:20 PM
💰We are very thankful to @kawresearch.bsky.social, @vetenskapsradet.bsky.social, SSFM, Kempe Stiftelserna, @mimsumea.bsky.social, @umeauniversitet.bsky.social, @qubelfastofficial.bsky.social, @wwiem-qub.bsky.social for support and helpful staff at @cp-cellreports.bsky.social!
Experiments 🔬were spearheaded by queen of illustrations 🎨 Sophie Tronnet (now building her group at @univ-amu.fr) with key computational work 🖥️ by Vikash Pandey in Oliver Billker’s lab, and great help from Miriam Lloret, Mario Pérez, Carlos Hernández, Niklas Söderholm, and Anders Nordström.
October 14, 2025 at 5:20 PM
Experiments 🔬were spearheaded by queen of illustrations 🎨 Sophie Tronnet (now building her group at @univ-amu.fr) with key computational work 🖥️ by Vikash Pandey in Oliver Billker’s lab, and great help from Miriam Lloret, Mario Pérez, Carlos Hernández, Niklas Söderholm, and Anders Nordström.
The response is concentration-, time-, and medium-dependent and several changes were induced by intact eATP. This implies that eATP sensing takes place at the bacterial surface. How? Some more🕵️♀️ detective work required there! 🔍 As always, stay tuned!
October 14, 2025 at 5:20 PM
The response is concentration-, time-, and medium-dependent and several changes were induced by intact eATP. This implies that eATP sensing takes place at the bacterial surface. How? Some more🕵️♀️ detective work required there! 🔍 As always, stay tuned!
In probiotic, pathobiontic, and pathogenic Enterobacteriaceae, eATP regulates fitness and virulence factors, supporting the notion that eATP could act as intestinal site- and infection stage-dependent cue🚦in bacteria causing high eATP (Shigella, Salmonella, EPEC...) or in bystanders.
October 14, 2025 at 5:20 PM
In probiotic, pathobiontic, and pathogenic Enterobacteriaceae, eATP regulates fitness and virulence factors, supporting the notion that eATP could act as intestinal site- and infection stage-dependent cue🚦in bacteria causing high eATP (Shigella, Salmonella, EPEC...) or in bystanders.
Good news for protective responses 🛡️against infection: eATP sensitises E. coli to antimicrobial peptides. Less good news for antibiotic therapy 💊: after eATP treatment, it can go either way.
October 14, 2025 at 5:20 PM
Good news for protective responses 🛡️against infection: eATP sensitises E. coli to antimicrobial peptides. Less good news for antibiotic therapy 💊: after eATP treatment, it can go either way.
And bacteria 🦠 respond to eATP, too! The response of non-pathogenic E. coli was sweeping, with 20% of the genome regulated downstream of eATP, induction of intracellular second messengers, and modulation of biofilm formation, metabolism, and cell envelope composition.
October 14, 2025 at 5:20 PM
And bacteria 🦠 respond to eATP, too! The response of non-pathogenic E. coli was sweeping, with 20% of the genome regulated downstream of eATP, induction of intracellular second messengers, and modulation of biofilm formation, metabolism, and cell envelope composition.
High eATP in the gut regulates inflammation and is triggered by infection.
doi.org/10.1016/j.im...
doi.org/10.1016/j.ce...
Since not only host cells but also gut microbes 🦠 are exposed to eATP,💡we wondered whether bacteria can respond to eATP fluctuations. Animals 🐕, plants 🌴, and fungi 🍄 do!
doi.org/10.1016/j.im...
doi.org/10.1016/j.ce...
Since not only host cells but also gut microbes 🦠 are exposed to eATP,💡we wondered whether bacteria can respond to eATP fluctuations. Animals 🐕, plants 🌴, and fungi 🍄 do!
Redirecting
doi.org
October 14, 2025 at 5:20 PM
High eATP in the gut regulates inflammation and is triggered by infection.
doi.org/10.1016/j.im...
doi.org/10.1016/j.ce...
Since not only host cells but also gut microbes 🦠 are exposed to eATP,💡we wondered whether bacteria can respond to eATP fluctuations. Animals 🐕, plants 🌴, and fungi 🍄 do!
doi.org/10.1016/j.im...
doi.org/10.1016/j.ce...
Since not only host cells but also gut microbes 🦠 are exposed to eATP,💡we wondered whether bacteria can respond to eATP fluctuations. Animals 🐕, plants 🌴, and fungi 🍄 do!
Many thanks also to local speakers Dessi Malinova, @selindaorrqub.bsky.social, @beckiejoingram.bsky.social, Ultan Power, @josebengoechea.bsky.social, @brendangilmore.bsky.social, co-organiser @valvanom.bsky.social, and chairs @colllab.bsky.social, @gunnarns.bsky.social, and @cisneroslab.bsky.social.
October 7, 2025 at 3:41 PM
Many thanks also to local speakers Dessi Malinova, @selindaorrqub.bsky.social, @beckiejoingram.bsky.social, Ultan Power, @josebengoechea.bsky.social, @brendangilmore.bsky.social, co-organiser @valvanom.bsky.social, and chairs @colllab.bsky.social, @gunnarns.bsky.social, and @cisneroslab.bsky.social.