Bob Kuhn
@kuhnbobio.bsky.social
High school biotech teacher at Innovation Academy HS Interested in Wolbachia, arthropod/microbe symbiosis, and soil phages. Currently studying sex ratio distorters in A vulgare pullbugs in the US. https://sites.google.com/view/room223/classroom-research
Coming soon. Want to contribute?
October 24, 2025 at 10:58 AM
Coming soon. Want to contribute?
Sampled the Winogradsky column today in 6 places and extracted DNA. Looking forward to seeing how much DNA we extracted and Nanopore 16S metabarcoding on Friday.
October 22, 2025 at 10:56 PM
Sampled the Winogradsky column today in 6 places and extracted DNA. Looking forward to seeing how much DNA we extracted and Nanopore 16S metabarcoding on Friday.
How your email finds me
September 30, 2025 at 8:00 PM
How your email finds me
Colors starting to emerge.
September 30, 2025 at 11:53 AM
Colors starting to emerge.
Column is starting to compact. Water rose and we took about a cup to filter for 16S Nanopore metabarcoding. Some brown spots emerging and bottom of column getting darker.
September 22, 2025 at 12:17 PM
Column is starting to compact. Water rose and we took about a cup to filter for 16S Nanopore metabarcoding. Some brown spots emerging and bottom of column getting darker.
Winogradsky column is go!
September 18, 2025 at 10:28 PM
Winogradsky column is go!
Almost done with the large Winogradsky column. We have some leakage but sealant should fix . The sampling ports went in well. Hopefully Thursday we can add our mud. 😀
September 13, 2025 at 5:09 PM
Almost done with the large Winogradsky column. We have some leakage but sealant should fix . The sampling ports went in well. Hopefully Thursday we can add our mud. 😀
I did 5 trials today using different Qiagen DNeasy Blood and Tissue extraction protocols for pillbug heads. Also did a Zymo bead bashing for comparison. Overall DNA yields from Qubit varied per method (mostly nuclear DNA) but CO1 PCR amplicons were similar across tests. One sample did not amplify.
August 23, 2025 at 10:04 PM
I did 5 trials today using different Qiagen DNeasy Blood and Tissue extraction protocols for pillbug heads. Also did a Zymo bead bashing for comparison. Overall DNA yields from Qubit varied per method (mostly nuclear DNA) but CO1 PCR amplicons were similar across tests. One sample did not amplify.
6 out of 11 student groups have phages. Time to try more soil so we can get all 11.
August 19, 2025 at 11:35 AM
6 out of 11 student groups have phages. Time to try more soil so we can get all 11.
Phage enrichment. First time scaling up for entire classes of students.
August 12, 2025 at 7:59 PM
Phage enrichment. First time scaling up for entire classes of students.
Salt shaker of death @lovettbr.bsky.social
August 11, 2025 at 10:20 PM
Salt shaker of death @lovettbr.bsky.social