Korostelev Lab
@korostelevlab.bsky.social
We are interested in life sciences, structural biology, translation, ribosomes, neurobiology and RNA therapeutics.
Location: RNA Therapeutics Institute, UMass Chan Medical School, Worcester, MA, USA
https://www.umassmed.edu/korostelev-lab/
Location: RNA Therapeutics Institute, UMass Chan Medical School, Worcester, MA, USA
https://www.umassmed.edu/korostelev-lab/
ROOL monomers entangle with other monomers via strand swapping to form octameric RNA nanocages.
www.nature.com/articles/s41...
www.nature.com/articles/s41...
June 16, 2025 at 6:03 PM
ROOL monomers entangle with other monomers via strand swapping to form octameric RNA nanocages.
www.nature.com/articles/s41...
www.nature.com/articles/s41...
With our @RTI-UMassChan.bsky.social colleagues Xiaobin Ling and Wenwen Fang, we report cryo-EM structures of enigmatic bacterial RNA nanocages made of ROOL RNAs. Octamers are held by "A-minor staples" and other interactions. Congrats Xiaobin, Dima et al!
www.nature.com/articles/s41...
www.nature.com/articles/s41...
June 16, 2025 at 6:01 PM
With our @RTI-UMassChan.bsky.social colleagues Xiaobin Ling and Wenwen Fang, we report cryo-EM structures of enigmatic bacterial RNA nanocages made of ROOL RNAs. Octamers are held by "A-minor staples" and other interactions. Congrats Xiaobin, Dima et al!
www.nature.com/articles/s41...
www.nature.com/articles/s41...
Activation of Retron-Septu for antiphage defense. Congrats
Tian-Min and all co-authors!
www.science.org/doi/full/10....
Tian-Min and all co-authors!
www.science.org/doi/full/10....
June 16, 2025 at 3:14 PM
Activation of Retron-Septu for antiphage defense. Congrats
Tian-Min and all co-authors!
www.science.org/doi/full/10....
Tian-Min and all co-authors!
www.science.org/doi/full/10....
RiSANA is online!
We detail the Ribosome Stimulated Angiogenin Nuclease Assay (RiSANA) to explore: (a) whether Angiogenin is activated by bacterial 70S ribosomes; and (b) whether the closest homolog RNase 4 is stimulated by 80S: tinyurl.com/3fftczrw
Congrats Emily Sholi and Anna Loveland!
We detail the Ribosome Stimulated Angiogenin Nuclease Assay (RiSANA) to explore: (a) whether Angiogenin is activated by bacterial 70S ribosomes; and (b) whether the closest homolog RNase 4 is stimulated by 80S: tinyurl.com/3fftczrw
Congrats Emily Sholi and Anna Loveland!
December 6, 2024 at 11:43 PM
RiSANA is online!
We detail the Ribosome Stimulated Angiogenin Nuclease Assay (RiSANA) to explore: (a) whether Angiogenin is activated by bacterial 70S ribosomes; and (b) whether the closest homolog RNase 4 is stimulated by 80S: tinyurl.com/3fftczrw
Congrats Emily Sholi and Anna Loveland!
We detail the Ribosome Stimulated Angiogenin Nuclease Assay (RiSANA) to explore: (a) whether Angiogenin is activated by bacterial 70S ribosomes; and (b) whether the closest homolog RNase 4 is stimulated by 80S: tinyurl.com/3fftczrw
Congrats Emily Sholi and Anna Loveland!
Activation of GCN2 relies on crossed helices at the dimer core: https://academic.oup.com/pnasnexus/advance-article/doi/10.1093/pnasnexus/pgae528/7906550
November 24, 2024 at 6:08 PM
Activation of GCN2 relies on crossed helices at the dimer core: https://academic.oup.com/pnasnexus/advance-article/doi/10.1093/pnasnexus/pgae528/7906550
Angiogenin controls angiogenesis (et plus) by nicking tRNAs. But it’s barely active alone. We discovered how 80S ribosome unlocks Ang’s active site and “brings in” tRNAs.
Congrats to Anna Loveland and collab. w/@ajplanetearth!
https://www.nature.com/articles/s41586-024-07508-8
Congrats to Anna Loveland and collab. w/@ajplanetearth!
https://www.nature.com/articles/s41586-024-07508-8
November 24, 2024 at 6:08 PM
Angiogenin controls angiogenesis (et plus) by nicking tRNAs. But it’s barely active alone. We discovered how 80S ribosome unlocks Ang’s active site and “brings in” tRNAs.
Congrats to Anna Loveland and collab. w/@ajplanetearth!
https://www.nature.com/articles/s41586-024-07508-8
Congrats to Anna Loveland and collab. w/@ajplanetearth!
https://www.nature.com/articles/s41586-024-07508-8
Congratulations to Dr. Christine Carbone on the 2023 @UMassChan Chancellor's thesis award! Her PhD work clarifies ribosome rescue (http://rdcu.be/b9EtA) and ribosome translocation, via time-resolved cryo-EM (http://rdcu.be/cC6E9).
Christine, congrats on your achievements!
Christine, congrats on your achievements!
November 24, 2024 at 6:08 PM
Congratulations to Dr. Christine Carbone on the 2023 @UMassChan Chancellor's thesis award! Her PhD work clarifies ribosome rescue (http://rdcu.be/b9EtA) and ribosome translocation, via time-resolved cryo-EM (http://rdcu.be/cC6E9).
Christine, congrats on your achievements!
Christine, congrats on your achievements!
Congrats to Dr. Christine Carbone on exemplar thesis defense!
Christine @seussofscience used cryo-EM to visualize ribosomal translocation by EF-G*GTP (https://rdcu.be/cC6E9) and ribosome rescue by ArfB (https://rdcu.be/b9EtA) – an exciting science journey full of surprises!
Christine @seussofscience used cryo-EM to visualize ribosomal translocation by EF-G*GTP (https://rdcu.be/cC6E9) and ribosome rescue by ArfB (https://rdcu.be/b9EtA) – an exciting science journey full of surprises!
November 24, 2024 at 6:08 PM
Congrats to Dr. Christine Carbone on exemplar thesis defense!
Christine @seussofscience used cryo-EM to visualize ribosomal translocation by EF-G*GTP (https://rdcu.be/cC6E9) and ribosome rescue by ArfB (https://rdcu.be/b9EtA) – an exciting science journey full of surprises!
Christine @seussofscience used cryo-EM to visualize ribosomal translocation by EF-G*GTP (https://rdcu.be/cC6E9) and ribosome rescue by ArfB (https://rdcu.be/b9EtA) – an exciting science journey full of surprises!
How do Dicer-1 and Loqs-PB make miRNA?
We captured 6 cryo-EM structures showing stages of pre-miRNA cleavage. Dicer•Loqs bend pre-miRNA to cleave the right targets:
https://www.cell.com/molecular-cell/fulltext/S1097-2765(22)00853-X
Fun collab. with @zamore_lab and Traci Hall...
We captured 6 cryo-EM structures showing stages of pre-miRNA cleavage. Dicer•Loqs bend pre-miRNA to cleave the right targets:
https://www.cell.com/molecular-cell/fulltext/S1097-2765(22)00853-X
Fun collab. with @zamore_lab and Traci Hall...
November 24, 2024 at 6:16 PM
How do Dicer-1 and Loqs-PB make miRNA?
We captured 6 cryo-EM structures showing stages of pre-miRNA cleavage. Dicer•Loqs bend pre-miRNA to cleave the right targets:
https://www.cell.com/molecular-cell/fulltext/S1097-2765(22)00853-X
Fun collab. with @zamore_lab and Traci Hall...
We captured 6 cryo-EM structures showing stages of pre-miRNA cleavage. Dicer•Loqs bend pre-miRNA to cleave the right targets:
https://www.cell.com/molecular-cell/fulltext/S1097-2765(22)00853-X
Fun collab. with @zamore_lab and Traci Hall...
Hot off the press: new review @AnnualReviews discusses recent time-resolved cryo-EM studies visualizing how ribosome moves and interacts with translation factors to achieve efficient and accurate translation.
“The Structural Dynamics of Translation”: https://tinyurl.com/2ezt9st9
“The Structural Dynamics of Translation”: https://tinyurl.com/2ezt9st9
November 24, 2024 at 6:16 PM
Hot off the press: new review @AnnualReviews discusses recent time-resolved cryo-EM studies visualizing how ribosome moves and interacts with translation factors to achieve efficient and accurate translation.
“The Structural Dynamics of Translation”: https://tinyurl.com/2ezt9st9
“The Structural Dynamics of Translation”: https://tinyurl.com/2ezt9st9
GGGGCC repeat expansion in C9ORF2 gene causes neurodegeneration in amyotrophic lateral sclerosis (ALS). The repeats produce peptides, such as poly-GR and poly-PR. We show that poly-GR/PR bind the ribosome peptide tunnel and block protein synthesis: https://rdcu.be/cNTwi
November 24, 2024 at 6:23 PM
GGGGCC repeat expansion in C9ORF2 gene causes neurodegeneration in amyotrophic lateral sclerosis (ALS). The repeats produce peptides, such as poly-GR and poly-PR. We show that poly-GR/PR bind the ribosome peptide tunnel and block protein synthesis: https://rdcu.be/cNTwi
Here is the second issue of articles and reviews dedicated to Alexander S. Spirin: https://link.springer.com/journal/10541/volumes-and-issues/86-9
Read up on informosomes, IRES RNAs and, of course, ribosomes.
Read up on informosomes, IRES RNAs and, of course, ribosomes.
November 24, 2024 at 6:23 PM
Here is the second issue of articles and reviews dedicated to Alexander S. Spirin: https://link.springer.com/journal/10541/volumes-and-issues/86-9
Read up on informosomes, IRES RNAs and, of course, ribosomes.
Read up on informosomes, IRES RNAs and, of course, ribosomes.
Two issues of commemorative articles and reviews are dedicated to Alexander S. Spirin, an outstanding scientist and visionary, who passed away last year.
Here is Part I on ribosomes, origin of life, RNA and more: https://link.springer.com/journal/10541/volumes-and-issues/86-8
Here is Part I on ribosomes, origin of life, RNA and more: https://link.springer.com/journal/10541/volumes-and-issues/86-8
November 24, 2024 at 6:30 PM
Two issues of commemorative articles and reviews are dedicated to Alexander S. Spirin, an outstanding scientist and visionary, who passed away last year.
Here is Part I on ribosomes, origin of life, RNA and more: https://link.springer.com/journal/10541/volumes-and-issues/86-8
Here is Part I on ribosomes, origin of life, RNA and more: https://link.springer.com/journal/10541/volumes-and-issues/86-8
mRNA frameshifting controls expression of some proteins; viruses use it to expand their coding repertoire. Our paper with @YaMingHou1 discusses +1 frameshifting as mRNA moves through the ribosome: https://www.nature.com/articles/s41467-021-24911-1
Congrats to G. Demo and all...
Congrats to G. Demo and all...
November 24, 2024 at 6:30 PM
mRNA frameshifting controls expression of some proteins; viruses use it to expand their coding repertoire. Our paper with @YaMingHou1 discusses +1 frameshifting as mRNA moves through the ribosome: https://www.nature.com/articles/s41467-021-24911-1
Congrats to G. Demo and all...
Congrats to G. Demo and all...
Ever wondered how the ribosome "hops" over a nucleotide to shift to another mRNA frame? We captured cryo-EM structures showing +1 frameshifting as EF-G translocates tRNA and mRNA. Check out preprint by Gabriel Demo et al; in collab with Ya-Ming Hou's lab:
http://disq.us/t/3tupvfl
http://disq.us/t/3tupvfl
November 24, 2024 at 6:30 PM
Ever wondered how the ribosome "hops" over a nucleotide to shift to another mRNA frame? We captured cryo-EM structures showing +1 frameshifting as EF-G translocates tRNA and mRNA. Check out preprint by Gabriel Demo et al; in collab with Ya-Ming Hou's lab:
http://disq.us/t/3tupvfl
http://disq.us/t/3tupvfl
Postdoc position open in @KorostelevLab, University of Massachusetts Medical School.
An opportunity to study translation, cell stress, neurobiology by cryo-EM, in situ EM, biochemistry.
For detail and application information:...
An opportunity to study translation, cell stress, neurobiology by cryo-EM, in situ EM, biochemistry.
For detail and application information:...
November 24, 2024 at 6:38 PM
Postdoc position open in @KorostelevLab, University of Massachusetts Medical School.
An opportunity to study translation, cell stress, neurobiology by cryo-EM, in situ EM, biochemistry.
For detail and application information:...
An opportunity to study translation, cell stress, neurobiology by cryo-EM, in situ EM, biochemistry.
For detail and application information:...
Ribosomes jam if mRNA is cut. But ArfB comes to rescue! It sneaks into mRNA tunnel and frees the peptide. We find that ArfB works on short and extended poly(A) mRNAs: here, ArfB dimer binds mRNA off the tunnel. See cryo-EM/biochem work by Carbone et al: https://rdcu.be/b9EtA
November 24, 2024 at 6:38 PM
Ribosomes jam if mRNA is cut. But ArfB comes to rescue! It sneaks into mRNA tunnel and frees the peptide. We find that ArfB works on short and extended poly(A) mRNAs: here, ArfB dimer binds mRNA off the tunnel. See cryo-EM/biochem work by Carbone et al: https://rdcu.be/b9EtA
Expanded GGGGCC repeats in the C9ORF72 gene cause amyotrophic lateral sclerosis and frontotemporal dementia. They produce toxic repeat proteins poly-PR and poly-GR. With Fen-Biao Gao's lab, we found how these peptides bind ribosomes and block translation:...
November 24, 2024 at 6:38 PM
Expanded GGGGCC repeats in the C9ORF72 gene cause amyotrophic lateral sclerosis and frontotemporal dementia. They produce toxic repeat proteins poly-PR and poly-GR. With Fen-Biao Gao's lab, we found how these peptides bind ribosomes and block translation:...
Nanoluciferase glows right upon release from the ribosome. This can be harnessed to study how ribosomes release complete or incomplete peptides (as in premature nonsense-codon diseases), and search for read-through drugs. See manuscript by Susorov et al:...
November 24, 2024 at 6:53 PM
Nanoluciferase glows right upon release from the ribosome. This can be harnessed to study how ribosomes release complete or incomplete peptides (as in premature nonsense-codon diseases), and search for read-through drugs. See manuscript by Susorov et al:...