(5/6) Moreover, we found that luminal pressure decreases and basal line tensions increase during budding, suggesting that budding has to be facilitated and stabilized by different mechanical processes.

(4/6) Looking at different time points, we found that apico-basal tension differences increase after 48h, when budding occurs, as expected. However, we also found that afterwards they decrease again, suggesting a ratchet-like irreversibility of budding.

(3/6) The method was verified with simulated data based on the bubbly vertex model.

(2/6) Image stacks of mouse intestinal organoids were segmented using the statistics of point clouds to obtain a fully 3D reconstruction of the cells, including all surface normals. Then force balance was used to infer surface and line tensions.

📝 New preprint! 📝

(1/6) Collaborative work between me and @omdrozdowski.bsky.social on 3D force inference for intestinal organoids is now on bioRxiv:

www.biorxiv.org/content/10.1...

From the labs of @ulrichschwarz.bsky.social and @michaelboutros.bsky.social .bsky.social with help from U. Engel.