Easy.
"Your plan was a failure Nai. Let's end this". I mean Again he said it so calmly while Knives went crazy with rage during the brawl! To me that speaks volumes about how the twins are with intense emotions. Vash is emotionally mature while Knives is unstable and childish

-Drum roll-

And my most favorite moment throughout the show and episode so far is....
The "It's over. Your plan was a failure Nai. This ends right now" scene! I mean...everything was perfect! Vash was so calm and mysterious and handsome too. The Studio even spiked his hair up for us fans Pure art!

Honorable mentions time! Other moments I loved but I didn't have enough room for this thread!

Knives going "i was rejected?" And "they are not your friends and they are not your family they're the enemy"

Vash going "because I'm vash the stampede" with the angel arm

Vash learning to fly

2. I adored everything about how both Myerl and the memory/ spirit of Rem helped Vash in the "Waking up" scene.
"Thank you Myerl I heard her voice through you". So cute and sweet. It made me cry before!
Rem really is with Vash always! Love and peace!

3. The short conversation Zazie had Wolfwood was fun as heck. I love how they have a sort of coworker relationship due to working for Knives. Good job voice actors