www.nature.com/articles/s41...

Thanks to all who made this possible! @eduardunterauer.bsky.social @evaschentarra.bsky.social @ipachmayr.bsky.social @taishatashrin.bsky.social Jisoo Kwon Sebastian Strauss, @jekristina.bsky.social @rafalkowalew.bsky.social @opazo.bsky.social @forna.bsky.social @lumasullo.bsky.social (6/6)

Within this neuronal atlas we can reveal the three synapse classes, excitatory, inhibitory and the recently discovered mixed synapse. Organelle imaging of Peroxisomes (Pmp70) and the Golgi Apparatus (Golga5) reveals rare contact sides and even fused particles. (5/6)

To show the power of the technique, we acquired a 13-plex 200 x 200 µm2 neuronal atlas in 3D. With this atlas we map the interaction architecture of three neurons, resolving organelles, cytoskeleton, vesicles and synapses at single-protein resolution. (4/6)

We demonstrate speed-optimized left-handed DNA-PAINT by characterizing the sequence binding kinetics and resolving three main microscopy benchmarking targets, mitochondria, microtubules and nuclear pore complexes with <5 nm localization precision. (3/6)

The mirrored design of left-handed oligonucleotides allows the extension of the common 6 speed-sequences R1-R6 with their analogs L1-L6, enabling 12 target multiplexing with a standard secondary label-free DNA-PAINT workflow. (2/6)

Congratulations to everyone involved: @ipachmayr.bsky.social, @lumasullo.bsky.social, @susannereinhardt.bsky.social, Jisoo Kwon, Ondřej Skořepa, Maite Llop, Sylvia Herter, Marina Bacac and Christian Klein. (6/6)
Read the full story here: www.nature.com/articles/s41...

The shift from Type II to Type I function reveals a structure–function continuum for anti-CD20 antibodies, showing that receptor arrangements dictate mechanism of action. RESI provides a platform for structure-guided antibody development, applicable far beyond CD20. (5/6)

We showed a direct link between CD20 oligomerization and function by investigating OBZ-based T-cell engagers (TCEs). An increased IgG flexibility in the 2+1 TCE format lead to increased CD20 tetramerization, without higher-order clustering, resulting in a reduction of direct cytotoxicity. (4/6)

In contrast, Type II antibodies like Obinutuzumab and H299 induced limited oligomerization to dimers, trimers and tetramers, consistent with their role in promoting direct tumor cell death rather than complement activation. (3/6)

By imaging intact cells, we could see these therapeutic antibodies in action: Type I antibodies like Rituximab and Ofatumumab formed extended chains of CD20 hexamers or larger, creating platforms compatible with complement protein binding, mediating cancer cell killing. (2/6)

@larissaheinze.bsky.social

@moniquehonsa.bsky.social , @philippsteen.bsky.social , Larissa Heinze, Shuhan Xu, Heinrich Grabmayr, Isabelle Pachmayr, Susanne C. M. Reinhardt, Ana Perovic, Jisoo Kwon, Ethan P. Oxley, Ross A. Dickins, Maartje M. C. Bastings, Ian A. Parish

Big congrats to @lumasullo.bsky.social and @rafalkowalew.bsky.social who led the project as well as other co-authors that contributed to this work!!

To facilitate SPINNA’s widespread use in the scientific community, we offer an open-source Python implementation and a GUI available in the latest version of Picasso (github.com/jungmannlab/..., picassosr.readthedocs.io/en/latest/sp...). 7/7

Finally, we investigate the dimerization of CD80 and PD-L1, key surface ligands involved in immune cell signaling. 6/7

We further quantitatively evaluate the oligomerization of the Epidermal Growth Factor Receptor (EGFR) upon binding of its ligand, EGF. 5/7