Great idea. Can you add me please.

🫣 I'm guilty of this in my latest Molecular Cancer paper! Many of our lncRNAs don't have GENCODE IDs so we kept the XLOC_IDs from the output of our custom assembly. But this will get confusing as inevitably there with be the same XLOC_IDs for 2 different lncRNAs produced from different assemblies.

With respect to (b) I can't help but wonder if this was observed because only high expressing lncRNAs (>5RPKM) were selected for the screens. LncRNAs working in cis are much more likely to be expressed at lower levels than those working in trans.

I’m not on the committee anymore and I don’t think they have migrated over yet. 😖

Hi. Would you mind adding me to the lncRNA starter pack please 🙏🏻

Could you add me to this starter please 🙏🏻 .

All good questions but not enough has been done in this area to answer them. There is some evidence of the formation of R loops which would confer specificity. Alternatively, the RNA could be inducing phase separation to concentrate TFs - doesn't account for the specificity though??

The lncRNA I’m working on is transcribed for an 11kb enhancer cluster. The RNA itself mediates the looping between the enhancer and the target gene and act of transcription maintains the 11kb H3K27Ac mark. The lncRNA also works in trans in the cytoplasm in the same pathway. Not just byproduct!

Hopefully see you there!