In an interview with Dr. Mishtu Dey, the editor-in-chief of Cell Chemical Biology, the authors of the Review entitled “Microbiota mechanisms in cancer progression and therapy” share their perspectives...

The worldwide spread of antibiotic resistance is considered to be one of the major health threats to society. While developing new antibiotics is crucial, there is also a strong need for next-generation analytical methods for studying the physiological state of live bacteria in heterogeneous populations and their response to environmental stress. Here we report a single-cell high-throughput method to monitor changes in the bacterial cell envelope in response to stress based on ratiometric flow cytometry. We used a sensitive fluorescent molecular probe, the Nile Red-based solvatochromic antimicrobial peptide UNR-1, with defined cellular localization in Gram-positive and Gram-negative bacteria. We also developed a robust protocol for calculating the generalized polarization (GP) of fluorescence adapted to flow cytometry. Our methodology enabled rapid detection of perturbations in the bacterial cell envelope caused by exposure to antibiotics, heat shock, and other factors.

Édition 2025 - Appel à candidatures La division SCF-ChemBio renouvelle l'attribution de son prix jeune chercheur/jeune chercheuse pour l'année 2025.

Modalités de candidature pour le prix de thèse 2024 SCF-ChemBio attribue un Prix de thèse pour l’année 2024. L’objectif est de récompenser des travaux de

Fluorescent probes for bacterial detection can be obtained by conjugating antimicrobial peptides with fluorescent dyes. However, little is known about the effect of the conjugation site and linker che...

After recent changes to Elon Musk’s X, a gradual migration turns into a stampede