Josie Gleeson
@josiegleeson.bsky.social
Postdoc in the Lappalainen lab at @scilifelab.se
RNA fangirl 👩🏼💻
RNA fangirl 👩🏼💻
No prob! Definitely doable, dRNA will be less biased than cDNA but may not get you the best depth. We had a post-mortem brain sample at RIN 7.8 and it still looked ok. I'd just advise using the RIN as a cofactor in analysis if you have varied samples.
June 4, 2025 at 11:17 AM
No prob! Definitely doable, dRNA will be less biased than cDNA but may not get you the best depth. We had a post-mortem brain sample at RIN 7.8 and it still looked ok. I'd just advise using the RIN as a cofactor in analysis if you have varied samples.
You can check out our paper here but we only went to a minimum of 7: doi.org/10.1093/narg.... Probably wouldn't advise doing it with much lower... depends what you want to see!
Pervasive effects of RNA degradation on Nanopore direct RNA sequencing
Abstract. Oxford Nanopore direct RNA sequencing (DRS) is capable of sequencing complete RNA molecules and accurately measuring gene and isoform expression.
doi.org
June 4, 2025 at 10:22 AM
You can check out our paper here but we only went to a minimum of 7: doi.org/10.1093/narg.... Probably wouldn't advise doing it with much lower... depends what you want to see!
Weird, for some reason I always thought amino acids would be way bigger!
March 21, 2025 at 1:50 PM
Weird, for some reason I always thought amino acids would be way bigger!