Journal of Orthopaedic Research - Spine®
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jorspine.bsky.social
Journal of Orthopaedic Research - Spine®
@jorspine.bsky.social
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JOR Spine is a fully open-access journal from @orssociety.bsky.social, dedicated to publishing high-quality spine research. https://onlinelibrary.wiley.com/journal/25721143?journalRedirectCheck=true
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Single-cell analysis integrated with machine learning elucidates the mechanisms of nucleus pulposus cells apoptosis in intervertebral disc degeneration and therapeutic interventions. Read more by Chao Song & coauthors: onlinelibrary.wiley....
Intersecting genes screening: (A). Intersecting Veen plot of Genecard data of IVDD with differential genes, (B). PPI network plot of intersecting genes, (C). Network plot of pivotal genes, and (D). Co-expression analysis of pivotal genes.
July 3, 2025 at 9:36 PM
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Daqian Zhou & coauthors performed an analysis of key sifferential metabolites in intervertebral disc degeneration based on untargeted metabolomics. Learn more in JOR Spine: onlinelibrary.wiley....
Differential metabolite analysis in different ways. (A) The matchstick plot of differential metabolites. (B)The radargram analysis of differential metabolites. (C) Correlation analysis of differential metabolites. (D) Chordal analysis of differential metabolites.
July 2, 2025 at 8:07 PM
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There is currently no consensus on the classification of lumbar facet joints based on X-ray imaging. Xiaowen Liu &coauthors developed and evaluated the reliability of a grading system: onlinelibrary.wiley....
Example diagram based on the X-ray grading system.
July 1, 2025 at 3:15 PM
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What is the significance of the MAPK signaling pathway in the diagnosis and subtype classification of intervertebral disc degeneration? Yong Liu & coauthors investigated this question: onlinelibrary.wiley....
The bioinformatics analysis workflow of this study is shown in Figure 1. We obtained 20 differentially expressed MAPK-related genes, of which RASGRP4, DUSP3, HSPA1A, HSPA6, IL1R1, KRAS, MAP3K5, RPS6KA1, BRAF, TNF, CACNA1I, and RPS6KA5 were overexpressed in IDD samples, whereas RASGRP1, DUSP14, DUSP2, RRAS2, JUN, PRKACB, RAP1A, and RAP1B were downregulated (Figure 2A,B). We researched the functions and molecular pathways of 20 differentially expressed MAPK-related genes in IDD. Biological process (BP) terms are related to Ras protein signal transduction, positive regulation of MAPK cascades, and regulation of ERK1 and ERK2 cascades; cellular component (CC) terms are related to ficolin-1-rich granule lumen, focal adhesion, and external side of plasma membrane; and molecular function (MF) terms are related to MAP kinase phosphatase activity, GDP binding, and G protein activity (Figure 2C Supplementary Table S2). KEGG terms are enriched mainly in MAPK signaling pathway, neurotrophin signal
June 30, 2025 at 1:11 PM
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This recent review by Matthew A. R. Trone & coauthors highlights hyperacidity's effects on the intervertebral disc, such as catabolism of disk cells, neoinnervation, altered mechanical signaling, and expression of pro-inflammatory cytokines and ion channels. Learn more: onlinelibrary.wiley....
Healthy versus degenerative intervertebral disk (IVD) and nerve ending. (A) Schematic showing a side-by-side comparison of a healthy versus degenerative IVD. (B) Schematic showing a side-by-side of a nerve ending innervating a healthy IVD versus a degenerative IVD, including mechanisms of low pH nociception. (C) Concentrations across the healthy IVD. Schematic showing pH and concentrations of oxygen and glucose across the disk from endplate to endplate in a healthy IVD. AF, annulus fibrosus; EPs, endplates; NP, nucleus pulposus.
June 8, 2025 at 11:06 PM
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Failure of spine implants is particularly serious due to the implant location near the spinal cord and nerves. Annette Kienle & coauthors suggest an improved approach to mechanical testing of new devices: onlinelibrary.wiley....
Step 1 of the literature-based approach to proof the mechanical safety of a spinal implant: Summary of the in vivo loading of the relevant spinal region and the implant under consideration of the intended use of the implant and human factors.
June 6, 2025 at 4:23 PM
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Posterior lumbar interbody fusion is used to treat various spine disorders, but can sometimes result in biomechanical deterioration in adjacent spine segments. Lipeng He & Tingchen Zhu and coauthors used finite element modeling to better understand this problem: onlinelibrary.wiley....
Nephograms of the stress distribution in the annulus under flexion loading conditions: Comparable stress values can be recorded in both Models 1 and Model 2, and a lower stress value can be observed in Model 3 and Model 4. Therefore, biomechanical significance of articular processes excision and preservation was limited. In contrast, obvious difference in stress values on the cranial motion segment can be observed between models with PLC excision and preservation.
June 4, 2025 at 8:02 PM
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A better understanding of different cell sub-populations present in the adult nucleus pulposus of spinal discs can provide insight into aging and degeneration. Andra-Maria Ionescu & coauthors present their findings in JOR Spine: onlinelibrary.wiley....
CD24, GD2 and TIE2 immunostaining in a cohort of pediatric and adult NP samples of a range of ages and degeneration grades. Representative images showing immunostaining for CD24 (A), GD2 (C) and TIE2 (E). Scale bars = 100 μm. For each marker, images represent (i) non-degenerate (grade 0), pediatric NP tissue (10 years old), (ii) mild degeneration (grade 4), adult NP tissue (28 years old); (iii) moderate degeneration (grade 7), elderly NP tissue (73 years old); (iv) severe degeneration (grade 12), elderly NP tissue (65 years old), (v) positive control (CD24: Colon; GD2: Breast carcinoma; TIE2: Testis) and (vi) IgG control (CD24: Colon; GD2: Breast carcinoma; TIE2: Testis). Quantification of marker positivity: (B) CD24, (D) GD2 and (F) TIE2, based on degeneration grade (B, D, F): Pediatric (degeneration grade = 0; n = 6), mild degeneration (2–5; n = 10), moderate degeneration (6–8; n = 10), severe degenerate (9–12; n = 10) and age (A, C, E): Pediatric (10–17, n = 6), young (20–35; n = 10
June 2, 2025 at 1:08 PM
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Chronic low back pain, a significant global health concern, is often linked to vertebral bone marrow lesions, such as fatty replacement. Wentian Li & coauthors explored relationships between the gut microbiome, serum metabolome, & fatty replacement in chronic low back pain: onlinelibrary.wiley....
A co-occurrence network constructed from the relative abundances of differential bacteria, and serum metabolites in LBP + FR/LBP subjects versus HCs.
May 23, 2025 at 12:21 PM
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Work by Ting-Yuan Tu & coauthors reveals how differences in extracellular matrix, stiffness, and myofibroblast distribution between ventral and dorsal layers unlock new insights into ligamentum flavum hypertrophy pathogenesis: onlinelibrary.wiley....
The experimental design of the study. (A) T2-weighted MRI image of hypertrophied LF at the L4/5 level, delineated by the red dotted line. Intraoperative photographs of hypertrophied ligamentum flavum specimens are categorized into ventral and dorsal segments based on their anatomical location. The upper image shows the dorsal LF specimen, the middle image displays the junction between the dorsal and ventral LF (yellow dashed line), and the lower image presents the ventral LF specimen (scale bar: 1 cm). (B) Experimental design of the study. Evaluation of ECM patterns using EVG staining through SEM to assess ECM composition and alignment, and AFM to measure mechanical stiffness. IHC staining was used to determine cell density and evaluate myofibroblasts, while protein expression of α-SMA was quantified using Western blot analysis. The LDH group served as the control, and comparisons were made between the LDH and LSS group, and within the LSS group between ventral and dorsal regions to as
May 21, 2025 at 11:50 AM
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Yangyang Xu & coauthors present a novel growth rod featuring unidirectional sliding and external regulation capabilities, providing dynamic correction and reducing the risk of infection through a non-invasive lengthening mechanism: onlinelibrary.wiley....
Experimental procedures.
May 20, 2025 at 1:56 AM
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Scoliosis instrumentation diminishes the natural coupling behavior of the spine. Could this explain correction loss and junctional deformity in the long-term? Read more from Christian Liebsch & coauthors: onlinelibrary.wiley....
Overview of the experimental setup including the spine tester (middle), the optical motion tracking system consisting of 12 infra-red cameras (right), and one of n = 6 thoracic and lumbar spine specimens (C7-sacrum) with rib cage equipped with three retroreflective markers at each vertebral level (left).
May 18, 2025 at 12:13 AM
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Aimin Gong, Daniel Yang, and Mengjie Zeng found genetic evidence supporting the causal relationship between arthritis and low back pain, especially highlighting the significant impact of hip osteoarthritis: onlinelibrary.wiley....
Flowchart of a Mendelian randomization study.
May 15, 2025 at 11:24 PM
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Work by Xiang-Yu Li & coauthors, employing multiomics approaches, revealed that the genes associated with lumbar disc degeneration are regulated by DNA methylation and identified genetic variances between gene expression and the gut microbiota: onlinelibrary.wiley....
Workflow of this study. A series of analyses were performed to identify candidate bone morphogenetic protein (BMP) genes associated with the pathogenesis of lumbar disc degeneration (LDD). BMP-related genes were extracted from the GeneCards database. Five transcriptome datasets, including LDD patients and healthy controls (HCs), were obtained from the GEO database, and a meta-analysis was conducted to ascertain the differential expression of LDD-associated BMP genes, followed by cell type-specific expression analysis (CSEA). A three-step summary data-based Mendelian randomization (SMR) method was used to integrate the genome-wide association study (GWAS) summaries and cis-eQTL (expression quantitative trait locus)/cis-mQTL (methylation quantitative trait locus) data from blood, and the putative blood BMP genes and their regulatory elements associated with LDD risk were prioritized (p-SMR-multi <0.01; HEIDI p > 0.05). Sensitivity analysis was performed after the initial SMR to test for
May 13, 2025 at 8:16 PM
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Can puerarin treat intervertebral disc degeneration by regulating nucleus pulposus cell death? Xiaoqiang Wang & coauthors present their findings in JOR Spine: onlinelibrary.wiley....
Molecular docking diagrams of puerarin with IL-1β, TNF-α, BCL2, Caspase3, and Caspase8. (A). Puerarin-IL-1β; (B). Puerarin-IL-1β; (C). Puerarin-BCL2; (D). Puerarin-Caspase3; D. Puerarin-Caspase8.
May 11, 2025 at 7:45 PM
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Explore how inflammatory cytokine Resistin (RETN) influences scoliosis progression - Muradil Mardan & coauthors found significant genetic links using bidirectional Mendelian randomization: onlinelibrary.wiley.... #ScoliosisResearch #Inflammation #Genetics #SpineHealth
The basic principle of bidirectional Mendelian randomization (MR) analysis.
May 9, 2025 at 6:18 PM
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Hanlong Xin & coauthors found that asymmetrical ESR1 expression of bilateral paraspinal muscle progenitor cells was correlated with asymmetrical paraspinal muscle size, asymmetrical fatty infiltration, and more severe scoliotic deformity: onlinelibrary.wiley....
Representative standard preoperative standing posteroanterior/lateral radiographs and MR imaging showing apical vertebra in major curve in ESR1 symmetry group (A) or ESR1 asymmetry group (B).
May 7, 2025 at 4:23 PM
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Recent work by Jie Yang & coauthors may help with understanding of the issues with unilateral lumbo-pelvic fixation and strategies for postoperative rehabilitation exercises in patients who has unilateral sacrum fracture: onlinelibrary.wiley....
A-P radiographs of pelvises with (A) bilateral lumbo‐pelvic fixation (BLF); (B) triangular osteosynthesis (TO); (C) unilateral lumbo‐pelvic fixation (ULF)
May 5, 2025 at 1:12 PM
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Stone Sima and Ashish Diwan discuss the contemporary clinical perspectives on chronic low back pain in their recent JOR Spine article. Read it here: onlinelibrary.wiley....
Contrasts a detached nucleus pulposus or contained disk herniation in a degenerated IVD compared to a healthy IVD. In the degenerated disk, there are many possible mechanisms of pain generation. (1) Damaged AF results in proliferation and activation of mechanoreceptors and nociceptors of the sinuvertebral nerve in the AF and nucleus pulposus. (2) Large, herniated disks can lead to mechanical compression and ischemia of surrounding neuronal structures. (3) Proinflammatory biomarkers like macrophages, TNF-a, C-reactive protein, and IL-1 are released as a response to the degenerative IVD and nerve damage.
April 27, 2025 at 2:57 PM
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Ruxolitinib delays nucleus pulposus cell senescence in rat intervertebral discs. Learn more in this recent JOR Spine article by Honggang Hao et al.: onlinelibrary.wiley....
Detection of NPC senescence rates by β-galactosidase staining. (A) Representative images of β-galactosidase staining; senescent NPCs are stained dark blue as SA-β-gal accumulates in lysosomes (scale bar: 50 μm). (B) Calculation of NPC senescence ratio = (number of senescent cells per unit field of view/total number of cells) × 100%. Data are presented as mean ± standard deviation from three independent experiments; *p < 0.05 versus control group, #p < 0.05 between two groups.
April 25, 2025 at 4:17 PM
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Shixiao Zhong & coauthors present their results on the clinical efficacy and biomechanical analysis of a novel hollow pedicle screw combined with kyphoplasty for the treatment of Kümmell disease: onlinelibrary.wiley....
(A) and (B) Axial and sagittal views of the T11-L2 3D model and T12 fracture model, respectively. (C) A compression fracture with intravertebral vacuum cleft model (M1). (D) A simple unilateral percutaneous kyphoplasty (PKP) model (M2). (E) A unilateral PKP combined with pediculoplasty model (M3). (F) A bilateral PKP combined with pediculoplasty model (M4). (G) A unilateral hollow pedicle screw combined with the kyphoplasty model (M5). (H) A bilateral hollow pedicle screw combined with the kyphoplasty model (M6). (I) Physical drawing of the novel hollow pedicle screw.
April 23, 2025 at 8:14 PM
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Check out Li Li & coauthor's recent paper to learn about the proteomic landscape of extracellular vesicles derived from human intervertebral disc cells: onlinelibrary.wiley....
Extracellular vesicle protein distribution in the Non-deg, Mildly-deg, and Deg samples. Venn diagram representing overlapping and unique proteins in EVs from IVD tissue, green, non-deg, orange, mildly-deg, and blue, Deg.
April 21, 2025 at 1:12 PM
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What are the effects of extracellular matrix‐degrading enzymes polymorphisms on intervertebral disc degeneration? Find out in this systematic review by Di Zhao & coauthors: pmc.ncbi.nlm.nih.gov...
Forest plot of rs243865 in MMP2 gene and the risk of IDD (CC/CT vs. TT). CI, confidence interval; IDD, intervertebral disc degeneration; M–H, Mantel–Haenszel.
April 19, 2025 at 4:41 PM
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A study of linezolid for intradiscal administration to treat chronic low back pain associated with Modic type 1 changes demonstrates consistent pharmacokinetics enabling selection of a dose regimen to assess efficay. Read more from Shiva S. Tripathi & coauthors: onlinelibrary.wiley....
Mean ± SD linezolid plasma concentrations of PP353 following a single intradiscal injection of 3 mL of PP353 to three subjects. 144 h timepoint is a mean of n = 2. The curve reaches a peak of 1000 ng/mL at < 24 hours and then declines to below 10 ng/mL by 144 hours.
April 17, 2025 at 2:12 PM
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An antibiotic formulation that can be injected into spinal discs to treat bacterial infection causing chronic low back pain is ready for first in human studies to assess safety and efficacy. Learn more about results from Graham Hagger et al.: onlinelibrary.wiley....
(A) Pharmacokinetics of PP353 in plasma and nucleus pulposus (mean ± SD). Linezolid concentrations in plasma after intradiscal (i.d.) administration of linezolid (5 mg/disc, 4 discs per animal) to sheep. Plasma concentrations over the first 24 h are mean of 6 animals and at 48 h 4 animals. The intradiscal concentrations are mean of 8 discs, 4 discs in each of 2 animals. (B) Plasma pharmacokinetics of linezolid after Intradiscal and intramuscular administration of PP353 (mean ± SD). Linezolid concentrations in plasma after intradiscal and intramuscular administration of linezolid (5 mg, 2 discs or 2 i.m. sites per sheep; 10 mg total) to sheep. Plasma concentrations up to 16 h are mean of 9 animals and beyond 16 h the mean of 6 animals.
April 15, 2025 at 12:13 PM