Joram Mooiweer
@jorammooiweer.bsky.social
Celiac disease mucosal immunity | Engineering intestine models | iPSCs, Organoids, T cells & organ-chips🔬| PhD candidate | Dept. of Genetics 🧬 | University Medical Center Groningen | University of Groningen (@rug.nl) |
📍Groningen, the Netherlands
📍Groningen, the Netherlands
Back to back commentaries in @natureportfolio.nature.com today. Clearly NAMs are causing a stir!
October 20, 2025 at 8:14 PM
Back to back commentaries in @natureportfolio.nature.com today. Clearly NAMs are causing a stir!
Every cell is a stem cell!
Even ‘terminal’ enterocytes can hit reset: Gut damage triggers a YAP/TAZ-driven fetal program → stress-proof “revival” stem cells → Lgr5⁺ CBC stem cells, rebuilding crypts from scratch and make organoids.
#StemCellPlasticity #Organoids #Gut
Even ‘terminal’ enterocytes can hit reset: Gut damage triggers a YAP/TAZ-driven fetal program → stress-proof “revival” stem cells → Lgr5⁺ CBC stem cells, rebuilding crypts from scratch and make organoids.
#StemCellPlasticity #Organoids #Gut
July 14, 2025 at 12:06 PM
Every cell is a stem cell!
Even ‘terminal’ enterocytes can hit reset: Gut damage triggers a YAP/TAZ-driven fetal program → stress-proof “revival” stem cells → Lgr5⁺ CBC stem cells, rebuilding crypts from scratch and make organoids.
#StemCellPlasticity #Organoids #Gut
Even ‘terminal’ enterocytes can hit reset: Gut damage triggers a YAP/TAZ-driven fetal program → stress-proof “revival” stem cells → Lgr5⁺ CBC stem cells, rebuilding crypts from scratch and make organoids.
#StemCellPlasticity #Organoids #Gut
Its honestly quite shocking.
Cancer cell lines are sub-optimal systems due to genomic instability, mutations and potentially strange phenotypes.
Thats known, but to see these anomalies quantified is a good moment to realize this once again.
#Celllines #ImmunoSky #Jurkat
Cancer cell lines are sub-optimal systems due to genomic instability, mutations and potentially strange phenotypes.
Thats known, but to see these anomalies quantified is a good moment to realize this once again.
#Celllines #ImmunoSky #Jurkat
July 4, 2025 at 6:29 AM
Its honestly quite shocking.
Cancer cell lines are sub-optimal systems due to genomic instability, mutations and potentially strange phenotypes.
Thats known, but to see these anomalies quantified is a good moment to realize this once again.
#Celllines #ImmunoSky #Jurkat
Cancer cell lines are sub-optimal systems due to genomic instability, mutations and potentially strange phenotypes.
Thats known, but to see these anomalies quantified is a good moment to realize this once again.
#Celllines #ImmunoSky #Jurkat
✨ Incredibly happy!!
To receive the 2025 Stimulering prize from the Dutch Coeliac Disease Association! 🙌
Huge Thanks to all NCV members for your trust💚
Excited to continue our mini-gut & gut-on-a-chip research at @umcgroningen.bsky.social 💚 #coeliakie #research #minigut
To receive the 2025 Stimulering prize from the Dutch Coeliac Disease Association! 🙌
Huge Thanks to all NCV members for your trust💚
Excited to continue our mini-gut & gut-on-a-chip research at @umcgroningen.bsky.social 💚 #coeliakie #research #minigut
July 2, 2025 at 9:28 PM
✨ Incredibly happy!!
To receive the 2025 Stimulering prize from the Dutch Coeliac Disease Association! 🙌
Huge Thanks to all NCV members for your trust💚
Excited to continue our mini-gut & gut-on-a-chip research at @umcgroningen.bsky.social 💚 #coeliakie #research #minigut
To receive the 2025 Stimulering prize from the Dutch Coeliac Disease Association! 🙌
Huge Thanks to all NCV members for your trust💚
Excited to continue our mini-gut & gut-on-a-chip research at @umcgroningen.bsky.social 💚 #coeliakie #research #minigut
Vascularized organoids!
Balancing BMP signaling enables co-differentiation of mesoderm + endoderm in a single spheroid → organ-specific endothelium + vascularized gut & lung organoids!
Ever increasing organoid complexity👏
#Organoids #StemCells
Balancing BMP signaling enables co-differentiation of mesoderm + endoderm in a single spheroid → organ-specific endothelium + vascularized gut & lung organoids!
Ever increasing organoid complexity👏
#Organoids #StemCells
June 30, 2025 at 9:36 PM
Vascularized organoids!
Balancing BMP signaling enables co-differentiation of mesoderm + endoderm in a single spheroid → organ-specific endothelium + vascularized gut & lung organoids!
Ever increasing organoid complexity👏
#Organoids #StemCells
Balancing BMP signaling enables co-differentiation of mesoderm + endoderm in a single spheroid → organ-specific endothelium + vascularized gut & lung organoids!
Ever increasing organoid complexity👏
#Organoids #StemCells
FDA 🤝 Organs-on-Chips
The @fda.gov recently published its Roadmap to Reducing Animal Testing in Preclinical Safety Studies. This is an exciting step forward in emphasizing the adoption of New Approach Methodologies (NAMs), focusing on human-derived in vitro and in silico models.
The @fda.gov recently published its Roadmap to Reducing Animal Testing in Preclinical Safety Studies. This is an exciting step forward in emphasizing the adoption of New Approach Methodologies (NAMs), focusing on human-derived in vitro and in silico models.
May 6, 2025 at 8:52 PM
FDA 🤝 Organs-on-Chips
The @fda.gov recently published its Roadmap to Reducing Animal Testing in Preclinical Safety Studies. This is an exciting step forward in emphasizing the adoption of New Approach Methodologies (NAMs), focusing on human-derived in vitro and in silico models.
The @fda.gov recently published its Roadmap to Reducing Animal Testing in Preclinical Safety Studies. This is an exciting step forward in emphasizing the adoption of New Approach Methodologies (NAMs), focusing on human-derived in vitro and in silico models.
Cheering for everyone standing up for science today!
👏👏👏
👏👏👏
March 7, 2025 at 8:41 PM
Cheering for everyone standing up for science today!
👏👏👏
👏👏👏
Seems obvious, but apparently, no one has really dived into it like this(?)👇
🧬 Epigenetic variation in iPSCs staged is mostly tied to genetic variation, but as cells differentiate, this dilutes out quickly: Cell type matters more than genetics.
#StemCells
www.nature.com/articles/s41...
🧬 Epigenetic variation in iPSCs staged is mostly tied to genetic variation, but as cells differentiate, this dilutes out quickly: Cell type matters more than genetics.
#StemCells
www.nature.com/articles/s41...
February 21, 2025 at 2:57 PM
Seems obvious, but apparently, no one has really dived into it like this(?)👇
🧬 Epigenetic variation in iPSCs staged is mostly tied to genetic variation, but as cells differentiate, this dilutes out quickly: Cell type matters more than genetics.
#StemCells
www.nature.com/articles/s41...
🧬 Epigenetic variation in iPSCs staged is mostly tied to genetic variation, but as cells differentiate, this dilutes out quickly: Cell type matters more than genetics.
#StemCells
www.nature.com/articles/s41...
“The world’s a shit show, so why not put 💩 on a chip?
Scientists created an iPSC-derived intestine-on-a-chip with fecal microbiota—uncovering epithelium-specific biomarkers & microbial factors linked to melanoma outcomes. Cutting-edge #OrganOnChip tech!
#ImmunoSky #MedSky”
Scientists created an iPSC-derived intestine-on-a-chip with fecal microbiota—uncovering epithelium-specific biomarkers & microbial factors linked to melanoma outcomes. Cutting-edge #OrganOnChip tech!
#ImmunoSky #MedSky”
February 14, 2025 at 9:46 AM
“The world’s a shit show, so why not put 💩 on a chip?
Scientists created an iPSC-derived intestine-on-a-chip with fecal microbiota—uncovering epithelium-specific biomarkers & microbial factors linked to melanoma outcomes. Cutting-edge #OrganOnChip tech!
#ImmunoSky #MedSky”
Scientists created an iPSC-derived intestine-on-a-chip with fecal microbiota—uncovering epithelium-specific biomarkers & microbial factors linked to melanoma outcomes. Cutting-edge #OrganOnChip tech!
#ImmunoSky #MedSky”
Its stories like this that makes me incredibly excited!
A gut immune–epithelial circuit regulating tolerance to food:
Goblet cell protein RELMβ disrupts tolerance by depleting microbiome-derived indoles needed for protective Tregs.
#microbiome #immunity #ImmunoSky
www.nature.com/articles/s41...
A gut immune–epithelial circuit regulating tolerance to food:
Goblet cell protein RELMβ disrupts tolerance by depleting microbiome-derived indoles needed for protective Tregs.
#microbiome #immunity #ImmunoSky
www.nature.com/articles/s41...
January 27, 2025 at 10:13 PM
Its stories like this that makes me incredibly excited!
A gut immune–epithelial circuit regulating tolerance to food:
Goblet cell protein RELMβ disrupts tolerance by depleting microbiome-derived indoles needed for protective Tregs.
#microbiome #immunity #ImmunoSky
www.nature.com/articles/s41...
A gut immune–epithelial circuit regulating tolerance to food:
Goblet cell protein RELMβ disrupts tolerance by depleting microbiome-derived indoles needed for protective Tregs.
#microbiome #immunity #ImmunoSky
www.nature.com/articles/s41...
TG2 (Transglutaminase 2) is responsible for the deamidation of gluten peptides, making them immunogenic antigens in the context of Celiac Disease - But what other functions does TG2 have?!
👇
TG2 drives epigenetic histone monoaminylation, regulating neural rhythmicity 🧠!
#Celiac
👇
TG2 drives epigenetic histone monoaminylation, regulating neural rhythmicity 🧠!
#Celiac
January 13, 2025 at 10:45 AM
TG2 (Transglutaminase 2) is responsible for the deamidation of gluten peptides, making them immunogenic antigens in the context of Celiac Disease - But what other functions does TG2 have?!
👇
TG2 drives epigenetic histone monoaminylation, regulating neural rhythmicity 🧠!
#Celiac
👇
TG2 drives epigenetic histone monoaminylation, regulating neural rhythmicity 🧠!
#Celiac
Time to log off for the holidays with a timelapse:
Intestinal organoid trying to resist the attack from CD8 T cells.
Happy holidays! 🎄🧑🎄
#ImmunoSky
Intestinal organoid trying to resist the attack from CD8 T cells.
Happy holidays! 🎄🧑🎄
#ImmunoSky
December 25, 2024 at 3:14 PM
Time to log off for the holidays with a timelapse:
Intestinal organoid trying to resist the attack from CD8 T cells.
Happy holidays! 🎄🧑🎄
#ImmunoSky
Intestinal organoid trying to resist the attack from CD8 T cells.
Happy holidays! 🎄🧑🎄
#ImmunoSky
Arrived. Excited for a 2-day Netherlands Organ-on-Chip Consortium gathering at the @hubrechtinstitute.bsky.social #Organoids #OrganOnChip #NOCI
December 18, 2024 at 8:48 AM
Arrived. Excited for a 2-day Netherlands Organ-on-Chip Consortium gathering at the @hubrechtinstitute.bsky.social #Organoids #OrganOnChip #NOCI
What do you find the most annoying piece of lab equipment and why is it the counting chamber?
#PhDchat #Immunosky
#PhDchat #Immunosky
December 5, 2024 at 3:11 PM
What do you find the most annoying piece of lab equipment and why is it the counting chamber?
#PhDchat #Immunosky
#PhDchat #Immunosky
The #intestine's dynamics (environment/movement/stiffness/flow) shapes stem cell fitness and function, shown here via PIEZO channels and is reflected in in-vitro models. So, are static 3D cultures sufficient? Could microphysiological systems #MPS #Organ-on-chip offer a better approach here?
December 1, 2024 at 10:22 AM
The #intestine's dynamics (environment/movement/stiffness/flow) shapes stem cell fitness and function, shown here via PIEZO channels and is reflected in in-vitro models. So, are static 3D cultures sufficient? Could microphysiological systems #MPS #Organ-on-chip offer a better approach here?
New #Preprint from INSERM Paris:
🔬Mucosa isolated #Tcells from #CrohnsDisease patients show increased interaction with AUTOLOGOUS(!) organoids, leading to:
- ⚡Enhanced epithelial differentiation
- 💥Increased epithelial cell death
- 🛡️Cytotoxic T cell activation
www.biorxiv.org/content/10.1...
🔬Mucosa isolated #Tcells from #CrohnsDisease patients show increased interaction with AUTOLOGOUS(!) organoids, leading to:
- ⚡Enhanced epithelial differentiation
- 💥Increased epithelial cell death
- 🛡️Cytotoxic T cell activation
www.biorxiv.org/content/10.1...
November 18, 2024 at 3:45 PM
New #Preprint from INSERM Paris:
🔬Mucosa isolated #Tcells from #CrohnsDisease patients show increased interaction with AUTOLOGOUS(!) organoids, leading to:
- ⚡Enhanced epithelial differentiation
- 💥Increased epithelial cell death
- 🛡️Cytotoxic T cell activation
www.biorxiv.org/content/10.1...
🔬Mucosa isolated #Tcells from #CrohnsDisease patients show increased interaction with AUTOLOGOUS(!) organoids, leading to:
- ⚡Enhanced epithelial differentiation
- 💥Increased epithelial cell death
- 🛡️Cytotoxic T cell activation
www.biorxiv.org/content/10.1...
How is there NO ONE at any pipette tips manufacturing company that comes up with the luminous idea to make the boxes for the p200 and p20 look only but a little tiny bit different!?
Again an aliquot that ended up in the p20 filter…😅
#ScienceSky #ImmunoSky
Again an aliquot that ended up in the p20 filter…😅
#ScienceSky #ImmunoSky
November 17, 2024 at 1:48 PM
How is there NO ONE at any pipette tips manufacturing company that comes up with the luminous idea to make the boxes for the p200 and p20 look only but a little tiny bit different!?
Again an aliquot that ended up in the p20 filter…😅
#ScienceSky #ImmunoSky
Again an aliquot that ended up in the p20 filter…😅
#ScienceSky #ImmunoSky
Nooit een betere gelegenheid gehad dan deze voor deze meme:
November 7, 2024 at 4:49 PM
Nooit een betere gelegenheid gehad dan deze voor deze meme:
Oh boy, #ICDS24 in Sheffield is very much on!
Incredible to see what has happened since last Celiac disease meeting. Much focus on Triggers, drivers, Viruses, B cells and more. It was an honor to give the scientific opening talk with the amazing @JonkersIris @ISSCD22
And this was only day 1…!
Incredible to see what has happened since last Celiac disease meeting. Much focus on Triggers, drivers, Viruses, B cells and more. It was an honor to give the scientific opening talk with the amazing @JonkersIris @ISSCD22
And this was only day 1…!
September 6, 2024 at 5:59 AM
Oh boy, #ICDS24 in Sheffield is very much on!
Incredible to see what has happened since last Celiac disease meeting. Much focus on Triggers, drivers, Viruses, B cells and more. It was an honor to give the scientific opening talk with the amazing @JonkersIris @ISSCD22
And this was only day 1…!
Incredible to see what has happened since last Celiac disease meeting. Much focus on Triggers, drivers, Viruses, B cells and more. It was an honor to give the scientific opening talk with the amazing @JonkersIris @ISSCD22
And this was only day 1…!
Intestinal epithelium and type-3 innate lymphoid cells (ILC3) bi-directionally regulate secretory dynamics and interleukin-22
www.mucosalimmunology.org/article/S193...
#Sciencesky
www.mucosalimmunology.org/article/S193...
#Sciencesky
December 18, 2023 at 10:11 PM
Intestinal epithelium and type-3 innate lymphoid cells (ILC3) bi-directionally regulate secretory dynamics and interleukin-22
www.mucosalimmunology.org/article/S193...
#Sciencesky
www.mucosalimmunology.org/article/S193...
#Sciencesky
biorxiv.org/content/10.110…
Interesting preprint reporting the Identification of a new LCN2+ NOX2+ DUOX2+ epithelial cell type expanded in IBD, 'specialised' in regulating mucosal immunology.
Interesting preprint reporting the Identification of a new LCN2+ NOX2+ DUOX2+ epithelial cell type expanded in IBD, 'specialised' in regulating mucosal immunology.
December 5, 2023 at 9:59 AM
biorxiv.org/content/10.110…
Interesting preprint reporting the Identification of a new LCN2+ NOX2+ DUOX2+ epithelial cell type expanded in IBD, 'specialised' in regulating mucosal immunology.
Interesting preprint reporting the Identification of a new LCN2+ NOX2+ DUOX2+ epithelial cell type expanded in IBD, 'specialised' in regulating mucosal immunology.
Today marks the opening of the MicrobiomeHub @umcg.
Im incredibly excited this unique opportunity to delve deeper into the #microbiome, #host-microbe interactions and eventually human health is now real. Onto much more groundbreaking health science!
@GroMicrobiome @researchumcg
Im incredibly excited this unique opportunity to delve deeper into the #microbiome, #host-microbe interactions and eventually human health is now real. Onto much more groundbreaking health science!
@GroMicrobiome @researchumcg
November 29, 2023 at 10:47 AM
Today marks the opening of the MicrobiomeHub @umcg.
Im incredibly excited this unique opportunity to delve deeper into the #microbiome, #host-microbe interactions and eventually human health is now real. Onto much more groundbreaking health science!
@GroMicrobiome @researchumcg
Im incredibly excited this unique opportunity to delve deeper into the #microbiome, #host-microbe interactions and eventually human health is now real. Onto much more groundbreaking health science!
@GroMicrobiome @researchumcg
Immunocompetent Human Intestinal #Organoids with autologous TISSUE RESIDENT immune cells! Deep characterization show maintenance of in-vivo phenotypes. The system also captures (drug induced) inflammation!
www.biorxiv.org/content/10.1...
www.biorxiv.org/content/10.1...
October 15, 2023 at 8:48 PM
Immunocompetent Human Intestinal #Organoids with autologous TISSUE RESIDENT immune cells! Deep characterization show maintenance of in-vivo phenotypes. The system also captures (drug induced) inflammation!
www.biorxiv.org/content/10.1...
www.biorxiv.org/content/10.1...