Jonathan Ready
@jonready.bsky.social
Use integrated methods (especially molecular tools) to map, track and model biodiversity and interactions in a quickly changing world. Based at UFPA, Belém, Brazil.
Now published. Local reference data is essential for detecting species of interest such as these critically endanged sawfishes.
www.sciencedirect.com/science/arti...
www.sciencedirect.com/science/arti...
Geographical variation in mitogenomes of the largetooth sawfish Pristis pristis: Challenges and perspectives for conservation efforts
Sawfishes (Pristidae) have been severely impacted by coastal development and unregulated fisheries and are considered Critically Endangered by the IUC…
www.sciencedirect.com
August 22, 2025 at 1:33 PM
Now published. Local reference data is essential for detecting species of interest such as these critically endanged sawfishes.
www.sciencedirect.com/science/arti...
www.sciencedirect.com/science/arti...
Reposted by Jonathan Ready
I also used a new method to quantify very long (2700 bp+) eDNA fragments using duplex ddPCR.
When we combine two mitochondrial assays in duplex, we can use double-positive droplets to quantify the "bridge" between them. Fancy but straightforward stuff.
Wanna know more? ask away :)
When we combine two mitochondrial assays in duplex, we can use double-positive droplets to quantify the "bridge" between them. Fancy but straightforward stuff.
Wanna know more? ask away :)
May 21, 2025 at 4:45 PM
I also used a new method to quantify very long (2700 bp+) eDNA fragments using duplex ddPCR.
When we combine two mitochondrial assays in duplex, we can use double-positive droplets to quantify the "bridge" between them. Fancy but straightforward stuff.
Wanna know more? ask away :)
When we combine two mitochondrial assays in duplex, we can use double-positive droplets to quantify the "bridge" between them. Fancy but straightforward stuff.
Wanna know more? ask away :)
Reposted by Jonathan Ready
🔬 Our new preprint is out!
We tracked the decay of multiple #eDNA 🧬& #eRNA molecules from bottlenose dolphins🐬 in seawater.
It may seem like I have caved in for charismatic megafauna. But do not worry. in reality, I am still only interested in their molecules.
+
www.biorxiv.org/content/10.1...
We tracked the decay of multiple #eDNA 🧬& #eRNA molecules from bottlenose dolphins🐬 in seawater.
It may seem like I have caved in for charismatic megafauna. But do not worry. in reality, I am still only interested in their molecules.
+
www.biorxiv.org/content/10.1...
Differential Decay of Multiple eNA Components from a Cetacean
Environmental nucleic acids (eNA), such as DNA and RNA, are powerful tools for monitoring biodiversity. Yet, interpreting eNA detections requires understanding their environmental persistence—especial...
www.biorxiv.org
May 21, 2025 at 4:45 PM
🔬 Our new preprint is out!
We tracked the decay of multiple #eDNA 🧬& #eRNA molecules from bottlenose dolphins🐬 in seawater.
It may seem like I have caved in for charismatic megafauna. But do not worry. in reality, I am still only interested in their molecules.
+
www.biorxiv.org/content/10.1...
We tracked the decay of multiple #eDNA 🧬& #eRNA molecules from bottlenose dolphins🐬 in seawater.
It may seem like I have caved in for charismatic megafauna. But do not worry. in reality, I am still only interested in their molecules.
+
www.biorxiv.org/content/10.1...
Important info for reporting standards in eDNA... onlinelibrary.wiley.com/doi/full/10....
eDNA Replicates, Polymerase and Amplicon Size Impact Inference of Richness Across Habitats
We conducted a large and controlled experiment, with bulk samples from terrestrial, freshwater and marine environments, to investigate the impacts of natural and technical replicate numbers, PCR poly....
onlinelibrary.wiley.com
May 9, 2025 at 4:33 PM
Important info for reporting standards in eDNA... onlinelibrary.wiley.com/doi/full/10....
Reposted by Jonathan Ready
New paper alert! 🚨- My final PhD chapter is finally out!
What happens to different eDNA components in water? 🌊💧
We tested particle size (small vs. large) and DNA molecule length (86 vs. 387 bp) in recirculating streams—and what we found might surprise you! 🧵👇
#eDNA #MolecularEcology
🔗 Read more:
What happens to different eDNA components in water? 🌊💧
We tested particle size (small vs. large) and DNA molecule length (86 vs. 387 bp) in recirculating streams—and what we found might surprise you! 🧵👇
#eDNA #MolecularEcology
🔗 Read more:
Comparing the Fate of eDNA by Particle Sizes and Molecule Lengths in Recirculating Streams
The detection of environmental DNA (eDNA) has revolutionized aquatic species monitoring, yet interpreting eDNA data remains challenging due to gaps in our understanding of eDNA ecology (i.e., origin,...
onlinelibrary.wiley.com
March 4, 2025 at 4:43 PM
New paper alert! 🚨- My final PhD chapter is finally out!
What happens to different eDNA components in water? 🌊💧
We tested particle size (small vs. large) and DNA molecule length (86 vs. 387 bp) in recirculating streams—and what we found might surprise you! 🧵👇
#eDNA #MolecularEcology
🔗 Read more:
What happens to different eDNA components in water? 🌊💧
We tested particle size (small vs. large) and DNA molecule length (86 vs. 387 bp) in recirculating streams—and what we found might surprise you! 🧵👇
#eDNA #MolecularEcology
🔗 Read more:
Local genetic reference sequences can be essential for species specific studies. Applying what worked for a species elsewhere in the world (in this case largetooth sawfish, Pristis pristis) may not work where you are!!! www.biorxiv.org/content/10.1...
Geographical variation in mitogenomes of the largetooth sawfish Pristis pristis: challenges and perspectives for conservation efforts
Sawfishes (Pristidae) have been severely impacted by coastal development and unregulated fisheries and are considered Critically Endangered by the IUCN Red List. Environmental DNA (eDNA) analyses have...
www.biorxiv.org
February 27, 2025 at 4:45 PM
Local genetic reference sequences can be essential for species specific studies. Applying what worked for a species elsewhere in the world (in this case largetooth sawfish, Pristis pristis) may not work where you are!!! www.biorxiv.org/content/10.1...
Reposted by Jonathan Ready
Proof that south american squid rings are not pig rectum!!!! doi.org/10.1038/s415...
Molecular identification of whole squids and calamari at fairs and markets in regions of Latin America - Scientific Reports
Scientific Reports - Molecular identification of whole squids and calamari at fairs and markets in regions of Latin America
doi.org
December 3, 2024 at 11:05 AM
Proof that south american squid rings are not pig rectum!!!! doi.org/10.1038/s415...
Reposted by Jonathan Ready
I'm hiring a coordinator for our #mesophotic Autonomous Reef Monitoring Structures (ARMS) program! This exciting new project will generate unprecedented amounts of information about the biodiversity of deep reefs in the Pacific. Spread the word! us242.dayforcehcm.co...
November 20, 2024 at 3:47 PM
I'm hiring a coordinator for our #mesophotic Autonomous Reef Monitoring Structures (ARMS) program! This exciting new project will generate unprecedented amounts of information about the biodiversity of deep reefs in the Pacific. Spread the word! us242.dayforcehcm.co...
Check out our new preprint investigating multiple factors that influence variation in outputs of eDNA metabarcoding analyses www.biorxiv.org/content/10.1...
November 20, 2024 at 4:42 PM
Check out our new preprint investigating multiple factors that influence variation in outputs of eDNA metabarcoding analyses www.biorxiv.org/content/10.1...