JLee Lab
@jleelab.bsky.social
Our lab at Baylor College of Medicine studies the logistics of cellular organization by focusing on membrane-bound and membrane-less organelle functions and contacts.
Lab lunch at Truth BBQ! A Houston classic!!
November 14, 2025 at 6:10 PM
Lab lunch at Truth BBQ! A Houston classic!!
A proper ☃️ for a legit snow day in HOUSTON!!? Mother Nature, never let them know your next move!
January 21, 2025 at 9:50 PM
A proper ☃️ for a legit snow day in HOUSTON!!? Mother Nature, never let them know your next move!
Gearing up for our lab’s holiday party! Grilling pizza, meats and veggies!!
December 19, 2024 at 5:42 PM
Gearing up for our lab’s holiday party! Grilling pizza, meats and veggies!!
8/P-bodies are thought to be sites of mRNA storage, however we see precise control of a key interaction for mRNA decapping. Are P-bodies multifunctional like most organelles? Can storage and decay both occur within P-bodies? Stay tuned for more work in this space!
November 13, 2024 at 2:55 PM
8/P-bodies are thought to be sites of mRNA storage, however we see precise control of a key interaction for mRNA decapping. Are P-bodies multifunctional like most organelles? Can storage and decay both occur within P-bodies? Stay tuned for more work in this space!
7/Not only are Dcp2-Dcp1A interactions diminished during stress, but they also do not return after 2-hrs of recovery even when stress granules have disassembled, and presumably when mRNA translation has resumed.
November 13, 2024 at 2:55 PM
7/Not only are Dcp2-Dcp1A interactions diminished during stress, but they also do not return after 2-hrs of recovery even when stress granules have disassembled, and presumably when mRNA translation has resumed.
6/Leyla only observed FRET shifts within P-bodies expressing Halo-Dcp1A (top). Thus, JF549 is a suitable FRET acceptor for mNeonGreen, and these data show Dcp2 and Dcp1A can interact within P-bodies. But what happens during stress?
November 13, 2024 at 2:55 PM
6/Leyla only observed FRET shifts within P-bodies expressing Halo-Dcp1A (top). Thus, JF549 is a suitable FRET acceptor for mNeonGreen, and these data show Dcp2 and Dcp1A can interact within P-bodies. But what happens during stress?
5/mNG-Dcp2 cells acutely expressing Halo-Dcp1A (top), or Halo-DDX6 (bottom-neg control). DonorOnly images were captured -> spike of JF549 -> recaptured same cells for FLIM-FRET. **Halo-expressing cells (green/magenta outlines). Non-Halo-expressing cells (white) served as an internal control.
November 13, 2024 at 2:55 PM
5/mNG-Dcp2 cells acutely expressing Halo-Dcp1A (top), or Halo-DDX6 (bottom-neg control). DonorOnly images were captured -> spike of JF549 -> recaptured same cells for FLIM-FRET. **Halo-expressing cells (green/magenta outlines). Non-Halo-expressing cells (white) served as an internal control.
4/Leyla cleverly thought to express mNeonGreen-Dcp2 as a donor together with an acceptor tagged with Halo, so a reference donor fluorescence lifetime could be captured in the absence of JaneliaFluor-549 ligand. How does it work?
November 13, 2024 at 2:55 PM
4/Leyla cleverly thought to express mNeonGreen-Dcp2 as a donor together with an acceptor tagged with Halo, so a reference donor fluorescence lifetime could be captured in the absence of JaneliaFluor-549 ligand. How does it work?
3/Around this time, Leyla Fahim joined the lab to develop FLIM-FRET for live tracking of interactions on ER membranes and within condensates, such as function-critical interactions between the decapping enzyme, Dcp2, and it's coactivator Dcp1A in P-bodies.
November 13, 2024 at 2:55 PM
3/Around this time, Leyla Fahim joined the lab to develop FLIM-FRET for live tracking of interactions on ER membranes and within condensates, such as function-critical interactions between the decapping enzyme, Dcp2, and it's coactivator Dcp1A in P-bodies.
2/The challenges of studying membrane-less condensates are rooted in the presence of condensate-enriched components within surrounding spaces. 1.5 years ago, Josh Marcus and I realized we could use filtered fluorescence lifetime phasor plots to separate stress granule pixels.
November 13, 2024 at 2:55 PM
2/The challenges of studying membrane-less condensates are rooted in the presence of condensate-enriched components within surrounding spaces. 1.5 years ago, Josh Marcus and I realized we could use filtered fluorescence lifetime phasor plots to separate stress granule pixels.
1/A Blutorial on our first paper published last month @jcellbiol.bsky.social. A picture is worth a thousand words but what if they can say more?! We developed live-cell FLIM approaches to track protein interactions within RNA-protein condensates. t.co/UF6T43xwqv
November 13, 2024 at 2:55 PM
1/A Blutorial on our first paper published last month @jcellbiol.bsky.social. A picture is worth a thousand words but what if they can say more?! We developed live-cell FLIM approaches to track protein interactions within RNA-protein condensates. t.co/UF6T43xwqv