Jim Hurley
@jimhurley.bsky.social
membranes, autophagy, lysosomes, cryo-EM and -ET, Parkinson's
Thrilled to join you and Claudine to study the cell's own version of Banksy's self-shredding artwork!
November 6, 2025 at 2:18 PM
Thrilled to join you and Claudine to study the cell's own version of Banksy's self-shredding artwork!
Animal cell biology per gpt5:
August 8, 2025 at 10:04 PM
Animal cell biology per gpt5:
Want to share your work at the premiere meeting of the minds between basic membrane biology and therapeutics? Short talk deadline is coming up Oct. 8, 2025. www.keystonesymposia.org/conferences/...
August 4, 2025 at 7:10 PM
Want to share your work at the premiere meeting of the minds between basic membrane biology and therapeutics? Short talk deadline is coming up Oct. 8, 2025. www.keystonesymposia.org/conferences/...
Congratulations @kev-in-situ-et.bsky.social and the whole team on in situ cryo-ET images of OA-induced mitochondria damage and Parkin-dependent mitophagy out now tinyurl.com/25b82cp4. We couldn't have done it without in situ guru @wilflinglab.bsky.social and mito guru @samlewis.bsky.social .
July 31, 2025 at 7:02 PM
Congratulations @kev-in-situ-et.bsky.social and the whole team on in situ cryo-ET images of OA-induced mitochondria damage and Parkin-dependent mitophagy out now tinyurl.com/25b82cp4. We couldn't have done it without in situ guru @wilflinglab.bsky.social and mito guru @samlewis.bsky.social .
And here's a bit of color. (I hate it when bsky inserts black boxes that you don't see till after its too late.)
July 25, 2025 at 8:12 PM
And here's a bit of color. (I hate it when bsky inserts black boxes that you don't see till after its too late.)
Structural and cell biology NPG publication two-fer today: lysosome signaling structural biology tinyurl.com/mt3uc96k with @robzonculab.bsky.social and mitophagy initiation tinyurl.com/3rpjyfjt with @martenslab.bsky.social @lazaroulab.bsky.social and @hummerlab.bsky.social
July 25, 2025 at 5:48 PM
Structural and cell biology NPG publication two-fer today: lysosome signaling structural biology tinyurl.com/mt3uc96k with @robzonculab.bsky.social and mitophagy initiation tinyurl.com/3rpjyfjt with @martenslab.bsky.social @lazaroulab.bsky.social and @hummerlab.bsky.social
In doing so, we also reproduced sharp peaks in the FSC at high spatial frequencies -often a sign of overfitting or artifacts when incorrect symmetry is applied.
May 20, 2025 at 8:58 PM
In doing so, we also reproduced sharp peaks in the FSC at high spatial frequencies -often a sign of overfitting or artifacts when incorrect symmetry is applied.
In our own analysis (tinyurl.com/66we8sn5), we found that in healthy cells, prohibitin is in a predominantly open, asymmetric state. Forcing 11-fold symmetry onto this heterogeneous population can produce densities resembling the published density and replicate the model of the 11-armed octopus.
May 20, 2025 at 8:58 PM
In our own analysis (tinyurl.com/66we8sn5), we found that in healthy cells, prohibitin is in a predominantly open, asymmetric state. Forcing 11-fold symmetry onto this heterogeneous population can produce densities resembling the published density and replicate the model of the 11-armed octopus.
To be convincing, a model should fill the density and should have physically plausible contacts between the subunits, which will hold the subunits in place, like this (rcsb.org 9O6S):
May 20, 2025 at 8:58 PM
To be convincing, a model should fill the density and should have physically plausible contacts between the subunits, which will hold the subunits in place, like this (rcsb.org 9O6S):
The Lange et al. paper presented a model of the prohibitin complex with 11 well-separated radial spokes, unlike any known structure of homologous proteins - a big warning sign. The shape was like an 11-armed octopus, with large gaps between the spokes and no physical mechanism holding them together.
May 20, 2025 at 8:58 PM
The Lange et al. paper presented a model of the prohibitin complex with 11 well-separated radial spokes, unlike any known structure of homologous proteins - a big warning sign. The shape was like an 11-armed octopus, with large gaps between the spokes and no physical mechanism holding them together.
mTORC1 is activated when cells are fed with amino acids. New preprint with @robzonculab.bsky.social led by Rachel Jansen shows how the massive "CastiGATOR" complex is formed when arginine-free CASTOR1 binds to the GATOR2 complex to antagonize mTORC1 starved cells. tinyurl.com/yr6en6f4
May 14, 2025 at 3:30 PM
mTORC1 is activated when cells are fed with amino acids. New preprint with @robzonculab.bsky.social led by Rachel Jansen shows how the massive "CastiGATOR" complex is formed when arginine-free CASTOR1 binds to the GATOR2 complex to antagonize mTORC1 starved cells. tinyurl.com/yr6en6f4
Absolutely! And, I recognize the jersey!
April 30, 2025 at 1:42 AM
Absolutely! And, I recognize the jersey!
A few favorite images:
March 25, 2025 at 6:25 PM
A few favorite images:
PINK1/Parkin-dependent mitophagy caught in situ by cryo-ET! New preprint www.biorxiv.org/content/10.1... led by @kev-in-situ-et.bsky.social. This has been a long-standing goal of team @mito911.bsky.social within the @asapresearch.parkinsonsroadmap.org initiative.
March 25, 2025 at 6:15 PM
PINK1/Parkin-dependent mitophagy caught in situ by cryo-ET! New preprint www.biorxiv.org/content/10.1... led by @kev-in-situ-et.bsky.social. This has been a long-standing goal of team @mito911.bsky.social within the @asapresearch.parkinsonsroadmap.org initiative.
Molecular dynamics by @hummerlab.bsky.social showed how the transition occurs and how the active state is aligned with the plane of the membrane to facilitate entry of the PI substrate lipid.
February 6, 2025 at 7:53 PM
Molecular dynamics by @hummerlab.bsky.social showed how the transition occurs and how the active state is aligned with the plane of the membrane to facilitate entry of the PI substrate lipid.
Therapeutic enhancement of autophagy may hold promise for Parkinson’s and other currently incurable neurodegenerative diseases. Targeting the VPS15 myristate could be an attractive path for therapeutic autophagy enhancement.
February 6, 2025 at 7:51 PM
Therapeutic enhancement of autophagy may hold promise for Parkinson’s and other currently incurable neurodegenerative diseases. Targeting the VPS15 myristate could be an attractive path for therapeutic autophagy enhancement.
The VPS15 pseudokinase binds to GTP, not ATP, thanks to an Arg residue at the gatekeeper position which is unique in the kinome. GTP binding controls the VPS34 conformation. @lazaroulab.bsky.social knocked out N-myristoylation and GTP binding, which dysregulates autophagy.
February 6, 2025 at 7:51 PM
The VPS15 pseudokinase binds to GTP, not ATP, thanks to an Arg residue at the gatekeeper position which is unique in the kinome. GTP binding controls the VPS34 conformation. @lazaroulab.bsky.social knocked out N-myristoylation and GTP binding, which dysregulates autophagy.
PI3KC3-C1 consists of the VPS34 lipid kinase, the pseudokinase VPS15, Beclin-1, and ATG14. Using cryo-EM, we found that N-myristoylation of VPS15 stabilizes the inactive conformation, which locks the VPS34 lipid kinase in an “off” state.
February 6, 2025 at 7:51 PM
PI3KC3-C1 consists of the VPS34 lipid kinase, the pseudokinase VPS15, Beclin-1, and ATG14. Using cryo-EM, we found that N-myristoylation of VPS15 stabilizes the inactive conformation, which locks the VPS34 lipid kinase in an “off” state.
Excited to see work led by @autophazeke.bsky.social, @minghaochen.bsky.social, Thanh Nguyen, and Ainara Claveras out in @science.org today, the culmination of a 15-year project to understand structurally how the synthesis of the critical lipid of autophagy, PI3P, is regulated. tinyurl.com/ywvjh8u4
February 6, 2025 at 7:51 PM
Excited to see work led by @autophazeke.bsky.social, @minghaochen.bsky.social, Thanh Nguyen, and Ainara Claveras out in @science.org today, the culmination of a 15-year project to understand structurally how the synthesis of the critical lipid of autophagy, PI3P, is regulated. tinyurl.com/ywvjh8u4
But it turns into a butterfly as soon as it departs, per Dorotea Fracchiolla.
November 17, 2024 at 5:04 PM
But it turns into a butterfly as soon as it departs, per Dorotea Fracchiolla.
Proud of new work tinyurl.com/yhz4c6ry from the lab by Zhicheng (Chen) Cui using single-particle cryo-EM on liposomes to show how mTORC1 integrates nutrient and growth factor signaling at lysosomes, collab. w/
G. Napolitano,A. Esposito, and A. Ballabio.
G. Napolitano,A. Esposito, and A. Ballabio.
November 16, 2024 at 4:31 PM
Proud of new work tinyurl.com/yhz4c6ry from the lab by Zhicheng (Chen) Cui using single-particle cryo-EM on liposomes to show how mTORC1 integrates nutrient and growth factor signaling at lysosomes, collab. w/
G. Napolitano,A. Esposito, and A. Ballabio.
G. Napolitano,A. Esposito, and A. Ballabio.
Join us @berkeleyMCB Mar. 25 for Cell Biology at the Nanoscale with @cellarchlab @bronwynalucas.bsky.social @thevillalab.bsky.social and more. Register at tinyurl.com/5n7jd7mr.
February 2, 2024 at 2:59 PM
Join us @berkeleyMCB Mar. 25 for Cell Biology at the Nanoscale with @cellarchlab @bronwynalucas.bsky.social @thevillalab.bsky.social and more. Register at tinyurl.com/5n7jd7mr.