Jeffrey C.-F. Huang
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jfhorner.bsky.social
Jeffrey C.-F. Huang
@jfhorner.bsky.social
A Taiwanese in Chicago 👨‍🔬📯🌈🧋🧋🧋
Proteome explorer, horn player and boba enthusiast!
Using bottom-up proteomics/antibodies, we confirmed that some phosphorylations appear constitutive, while others are more sensitive to myosin inhibition, raising the possibility of a mechano-sensitive “phospho-code” for cell-cell adhesion. (5/7)
October 31, 2025 at 8:01 PM
Remarkably, catenin phosphorylation is sensitive to drugs that modulate #actomyosin contractility. We observed HYPER-phosphorylation on catenins under conditions that promote cell rounding and HYPO-phosphorylation when myosin activity was reduced. (4/7)
October 31, 2025 at 8:01 PM
With Team @carajgottardi.bsky.social ‘s expertise in cell-cell adhesion, we purified cadherin-associated catenins and profiled their proteoforms using I2MS and found #phosphorylation to be the MAJOR modification. (3/7)
October 31, 2025 at 8:01 PM
Team @nlkproteomics.bsky.social has developed individual ion mass spectrometry (I2MS) for proteoform analysis. We extend the current ~70 kDa mass limit beyond 100 kDa in this work. (2/7)
October 31, 2025 at 8:01 PM
Heading to #HUPO2025! I’ll talk about our catenin phospho-code story and how top-down MS reveals how adhesion proteins respond to actomyosin force!

Thanks for the invite, #AFFINISEP!

Full story on bioRxiv 👉https://doi.org/10.1101/2025.09.06.674621
October 27, 2025 at 3:44 PM
The mass spectrometry research community at Chicago is thriving! Many thanks to the Chicago Mass Spec Interest Group for the invitation to share my catenin proteoform story in the inaugural event. It was a pleasure to meet with researchers at the beautiful CZbiohub space!
April 11, 2025 at 4:59 PM
Looking forward to sharing my work at the Chicago Mass Spec Interest Group’s inaugural meeting!
April 5, 2025 at 7:18 PM
It was a very fruitful #USHUPO2025 ! I was beyond thrilled to share our advances in catenin proteoform profiling and functional studies that will reveal the phospho-code in cell-cell adhesions. Many thanks to the conference organizers and participants!
February 27, 2025 at 4:05 AM
Looking forward to attending the #USHUPO conference in Philly this weekend and sharing some early results in catenin proteoform profiling and our vision to build a story behind acto-myosin contractility regulation in cell-cell adhesion. Please join my talk next Wednesday and say hi!
February 17, 2025 at 9:39 PM
It was a tremendous pleasure to visit my alma mater National Taiwan University and give a talk on top-down proteomics and proteoform biology at the Institute of Biomedical Sciences earlier this week.
December 29, 2024 at 7:56 AM
Going home from the lab be like 🥶. First snow is Chicago!
November 21, 2024 at 3:12 AM
I’m so glad being in Chicago where I get to enjoy the finest musicians playing in concerts. Photo from Daniil Trifonov’s piano recital this afternoon at Chicago Symphony Orchestra!
November 18, 2024 at 4:55 AM