Awhh, my lab are absolute legends!

We are looking to take on an intern at EMBL Rome, for anyone interested in CRISPR and Epigenetics training.

The traineeship is 3-12-months, in Rome (Italy), should start by July, and is supervised by the outstanding @steliostsagkris.bsky.social.

#job #internship; see info below...

Postdoc positions in epigenetics!

We have several opportunities to investigate big picture questions on (epi)genome function & mechanisms

Based @embl.org near Rome (Italy), we offer full scientific support/facilities, training & funding. Also...☀️🏛️🍕🏖️

See👇or contact me for other options. Pls RS

Finally, what about aging? Well that certainly has an effect.

We detected strong patterns of altered gene expression as early as blastocysts when comparing embryos derived from young vs mature fathers 11/

Using this metric we found paternal conditioning increase variation in expression across the F1 population.

This could be an important phenotypic readout and explain observations of partial penetrance in intergenerational postnatal phenotypes 10/

Rather than traditional DEG calling - that looks for consistent changes - we also asked whether fathers induce excess variation in expression amongst early embryos 9/

Pushing further down this line, changing the paternal genetic background completely alters (or ablates) the nature of intergenerational response seen in F1 embryos.

This implies a sort of intergenetional GxE (iGxE) interaction that should be further considered 8/

This effect appears to be robust over independent batches, e.g. Gata4 below (each datapoint = a blastocyst)

But! many genes/pathway effects are NOT batch-robust. Thus, multiple batches and independent replicates are necessary to tease out noise/confounders in intergenerational studies 7/

What is the signature? It seems different expsoures have different effects.

Paternal dybiosis by antibiotics (nABX) appears to affect lineage marker expression and/or allocation of the primitive endoderm lineage relative to epiblast lineage

This is not seen in paternal LPHS embryos 6/

Indeed, using many replicates/batches, we detected induction of a clear gene expression signature in blastocysts, depending on paternal conditioning 5/

Clustering on transcriptome showed no separation. Not surprising since these embryos should all develop into relatively normal pups

BUT, there was tendency for embryos to sit at opposite ends of the PC1 spectrum if their father was dysbiotic (nABX) vs on High-sugar (LPHS) diet. See histogram 4/

We used IVF at scale, with fathers exposed to gut dysbiosis, altered diets, or control used as sperm donors.

We then generated single-embryo transcriptomes from multiple batches and >800 embryos across the study 3/

Back at the EMBL mothership this week for the Quantitative Biology symposium

Looks a cracking speaker lineup and set of posters on diverse topics… and it’s definitely fresh in Heidelberg 🥶

www.embl.org/about/info/c...

Fantastically engaging talk on #microbiome -brain axis from @jfcryan.bsky.social in Rome this morning.

Full of thought-provoking ideas and some sage advice:

“If you’re thinking of having a midlife crises… forget about the motorbikes and think about leeks instead” 😂