Objectives 1. To develop a deep-learning segmentation model for automated measurement of maximal aortic diameter (Dmax) and volumes of aortic dissection components: true-lumen (TL), circulating false-...

Parallel magnetic resonance imaging (pMRI) reduces MRI acquisition time, with Sensitivity Encoding (SENSE) being a widely used method that exploits coil sensitivity maps for efficient image reconstru...

Abstract. The printing and dyeing industry is one of the most polluting (ca. 20% of global clean water pollution), water-consuming and energy-wasting secto

The biological role of DNA and RNA G-quadruplexes (G4s) is relayed to the cellular circuitry by a plethora of proteins known as G4-binding proteins (G4PBs). It is thus critical to decipher the formation of the G4/G4BP complexes to accurately understand their involvement in G4-mediated regulatory mechanisms. While hundreds of G4-interacting compounds (G4 ligands) have been used to uncover G4 biology in a rather indirect manner, only a handful of multivalent ligands allowing for identifying associated proteins have been designed, mostly relying on the covalent capture of G4BPs upon photoactivation. We report herein on such a multivalent ligand named photoMultiTASQ, which belongs to a family of biomimetic and smart G4 ligands known as template-assembled synthetic G-quartets (TASQs). We show how photoMultiTASQ interacts with and photo-cross-links both DNA/RNA G4s and related G4BPs, and develop a new mass spectrometry (MS)-based technique to characterize the covalent adducts. Collectively, these results make photoMultiTASQ a new molecular tool in the arsenal of chemical cross-linking and isolation by pull-down (Chem-CLIP) technologies that uniquely identify targets and validate target engagement in cells.

The potential of clustered regularly interspaced short palindromic repeats (CRISPR) and corresponding CRISPR-associated (Cas) protein systems (CRISPR/Cas) systems for biomedical applications is tremendous; however, precise control of their activity is essential to better harness this potential and, beyond this, to develop reliable diagnostic reagents. Herein, we report on such a strategy by controlling the CRISPR/Cas12a activity using a photo-controllable CRISPR RNA (crRNA). To this end, the 3′ end of crRNA was conjugated to a G-quadruplex (G4) block through a photocleavable linker: upon photo irradiation, the G4 trigger is removed, thus allowing for the DNA target to access and hybridize with the crRNA, and thus be processed by the CRISPR/Cas12a system. The efficiency of this approach was demonstrated by the detection of human papillomavirus 16 DNA in 50 clinical samples: our one-pot strategy was found to be as efficient as the routinely implemented method (qPCR), with 95.7% sensitivity and 100% specificity, in addition to be faster (25 versus 60 min) and both simpler and less expensive (being implementable as lateral flow test strips). Collectively, this new and fully controllable CRISPR/Cas system holds great potential for next-generation clinical diagnostics.

We report the synthesis and evaluation of the first 3,6,10,13-tetraaza-1,8(2,6)-dipyridinacyclotetradecaphane-3,6,10,13-tetraacetic acid (PYTA) bifunctional chelators (BFCs) for 225Ac coordination. Me...

Monoclonal antibodies have proven to be highly effective for in vivo targeting in nuclear medicine applications. However, typical radiolabeling methods, for PET/SPECT imaging and targeted radionuclide...

Objectives To assess 12-month outcomes of prostate artery embolization (PAE) using N-butyl cyanoacrylate (NBCA) glue in patients with incapacitating lower-urinary-tract symptoms (LUTS) due to benign p...

Colistin (COL) is a widely used antibiotic and is quite often used as a last-resort treatment option for treating multidrug-resistant Gram-negative bacterial infections. Due to its widespread use, COL...

Background Non-coding RNAs (ncRNAs) in human cells constitute a substantial portion of the transcriptome but do not lead to protein synthesis. Among them, long non-coding RNAs (lncRNAs, > 200 nucleoti...

The development of new fluorophores emitting in the near-infrared (NIR-I and/or NIR-II) region is currently a major focus of research in fields such a…

Pro-apoptotic receptor activators (PARAs), including second-generation agonist antibodies and ligands targeting the TNF receptor superfamily such as T…

Citation metrics are widely used and misused. We have created a publicly available database of top-cited scientists that provides standardized information on citations, h-index, co-authorship adjusted...