No lies detected

First part is a biophysical primer for a wide audience. Second is a recap of recent findings using ctenophores with @stevehaddock.bsky.social. Third is a re-analysis of published datasets to test between homeoviscosity and homeocurvature models. More soon!

Follow up to Keller et al 1993!

Definitely not dependent on FPs. Can be observed with solvatochromic dyes like Laurdan

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Wow

Awesome. The Elyra system is very powerful. We haven't run yeast on them yet, but looks compare to CLSM/Airyscan

Getting lectured by Markus Deserno about publishing bangers, now that’s rich!

Congrats to you Roberto!

Thanks Andrew!

Thanks André :)

This work was aided greatly through help from the Devaraj (UCSD), Obara (UCSD), and Best (UT Knockville) labs. It was supported by NIGMS and Paul Allen Family Foundation @alleninstitute.org. Reagents for implementing FACES will soon be available from Avanti Polar Lipids and Addgene.

We think FACES is a powerful approach for beginning to interrogate the hidden heterogeneity and asymmetry of intracellular membranes. It can also be applied to other molecules accessible to biorthogonal labeling, like intracellular glycans that are difficult to image via direct fluorescence.

He then shows that fusing fluorogen sensors to either side of a transmembrane domain allows for imaging of leaflet-specific phospholipid distributions in the trans-Golgi network. He sees that asymmetry is generated at TGN vesicles and finds transporters and flippases that control this asymmetry.

Will shows that organelle targeted FAPs can be used to untangle the role of lipid transfer proteins in trafficking of phospholipids like PC across organelle contact sites, like those of the ER and mitochondria.

FACES is the brainchild of incredibly creative and talented post-doc Will Moore @moorrhizal.bsky.social. It combines bioorthogonal labeling of phospholipids with fluorogen technology, in which fluorescence is generated upon coincidence of a lipid-bound dye and a genetically-encoded protein sensor.