This collaborative project was conducted across the following institutes. @stowersinstitute.bsky.social @mdanderson.bsk.social @oxfordwomenshealth.bsky.social @cambiochem.bsky.social @ox.ac.uk @cam.ac.uk

Thank you coauthors @shylonatasha.bsky.social @zgrif26.bsky.social @thomsanger.bsky.social @hollfelderlab.bsky.social @swilliamslabox.bsky.social @aussiebiologist.bsky.social @rrbehringer.bsky.social

Through confocal imaging we describe basement membrane deposition in the ovary during folliculogenesis. We can report a double basement membrane deposited on either side of the granulosa cell layer surrounding the follicles.

Exploring the sub-cellular architecture of the germinal bed & stage II follicles, we could analyse the granulosa (g) and theca (th) cell layers surrounding stage II follicles. Primordial follicles (pf) start exhibiting enlarged nuclei and cytoplasm!

Oogenesis takes place in 10 stages. It starts in the oogonial nest - a mitotic stem cell niche within the germinal bed - which then undergo meiosis to form primordial follicles
We identified oogonia and primordial follicles using section H&E staining paired with confocal DAPI/WGA staining!

i was told a US donor site to support FlyBase will be live shortly, which we should all broadcast to everyone we can. however, there is a delay (red tape) to be able to provide invoices to bill grants, but its in process. 🪰