HC1 is capable of tolerating low oxygen levels. In contrast, SC1 appears unable to do so, likely because it lacks genes related to oxygen metabolism. We believe this difference stems from the fact that SC1 was cultured from the beginning under strictly anaerobic conditions with reducing agents.

Thank you again for introducing our work!
I'd greatly appreciate it if you could correct one point in episode #4.

Lokiarchaeia is MBG-B, not MBG-D.

We have also published a paper on how to construct and operate a DHS bioreactor and isolate anaerobic microorganisms.
www.nature.com/articles/s41...

MK-D1 was not isolated from this bioreactor. The correct reference is as follows.
journals.plos.org/plosone/arti...

MK-D1 was isolated from marine sediment collected from a methane seep approximately 2500 meters deep in the Nankai Trough off the Kii Peninsula in Japan, not from a drill core sample collected off the Shimokita Peninsula.

Thank you so much for introducing our work!
Could you kindly make the following corrections to story #1?

Thank you for the kind comments!

Thank you for the kind comment!

Thank you!

I’m currently trying to isolate uncultivated deep-sea sedimentary microorganisms using the reactor enrichment as an inoculum. If you happen to have time, I would be honored if you could take a look at a paper I published two years ago, where I provide more details about the reactor.

I’m truly grateful for the warm welcome—thank you so much!

I apologize for the confusion. To clarify, I’ve been supplying 0.57L of anaerobic medium to the reactor each day, which brings the total to 3733L so far. Also, my reactor will be turning 18 on December 28th.