I am also really glad that people far more creative than I dressed up a hamster as Godzilla so that when I decided to name the cell line CHO ZeLa (for Zero Lacate) I could use lots of fun pictures for presentations.

We later made a range of knockouts and showed that knocking out even 1 (of 4) pyruvate dehydrogenase kinases enabled the knockout of Ldha and elimination of the Warburg effect. These cells grew as well as wildtype, did not produce lactate, and consumed much less glucose.

We first generated knockouts of all pyruvate dehydrogenase kinases and Ldha. We confirmed that lactate dehydrogenase is not detected, that cells do not produce lactate, and show negligible lactate dehydrogenase activity (wildtype in blue, knockout lines in red).

I am also really glad that people far more creative than I dressed up a hamster as Godzilla so that when I decided to name the cell line CHO ZeLa (for Zero Lacate) I could use lots of fun pictures for presentations.

Finally, we grew cells with Ldha and 1 (purple), 2 (green), 3 (orange), and 4 (red) pyruvate dehydrogenase kinases knocked out for ~3 months and showed that the Warburg-null phenotype was stable over time.

We later made a range of knockouts and showed that knocking out even 1 (of 4) pyruvate dehydrogenase kinases enabled the knockout of Ldha and elimination of the Warburg effect. These cells grew as well as wildtype, did not produce lactate, and consumed much less glucose.

We first generated knockouts of all pyruvate dehydrogenase kinases and Ldha. We confirmed that lactate dehydrogenase is not detected, that cells do not produce lactate, and show negligible lactate dehydrogenase activity (wildtype in blue, knockout lines in red).