Hoong Chuin Lim
@hclim.bsky.social
Congrats to Apurva, Brian and Irem and a big thank you to our collaborators:
• Brooke of the Kim lab (UIUC) for microscopy
• Molly of the Helaine lab (Harvard Med) for tRNA-seq
• Eric of the Corrêa lab (NEB) for cleavage site mapping by mass spec
• Brooke of the Kim lab (UIUC) for microscopy
• Molly of the Helaine lab (Harvard Med) for tRNA-seq
• Eric of the Corrêa lab (NEB) for cleavage site mapping by mass spec
September 15, 2025 at 12:56 AM
Congrats to Apurva, Brian and Irem and a big thank you to our collaborators:
• Brooke of the Kim lab (UIUC) for microscopy
• Molly of the Helaine lab (Harvard Med) for tRNA-seq
• Eric of the Corrêa lab (NEB) for cleavage site mapping by mass spec
• Brooke of the Kim lab (UIUC) for microscopy
• Molly of the Helaine lab (Harvard Med) for tRNA-seq
• Eric of the Corrêa lab (NEB) for cleavage site mapping by mass spec
Key highlights:
• A phage-inspired genetic screen to identify the targets of tRNA nucleases.
• OLD severs the anticodon stem with a one-base staggered cut.
• tRNAs destroyed by OLD are irreparable by T4 RNA ligase. • Both cuts occur after the first cytosine within a paired CNG pseudo-palindrome.
• A phage-inspired genetic screen to identify the targets of tRNA nucleases.
• OLD severs the anticodon stem with a one-base staggered cut.
• tRNAs destroyed by OLD are irreparable by T4 RNA ligase. • Both cuts occur after the first cytosine within a paired CNG pseudo-palindrome.
September 15, 2025 at 12:56 AM
Key highlights:
• A phage-inspired genetic screen to identify the targets of tRNA nucleases.
• OLD severs the anticodon stem with a one-base staggered cut.
• tRNAs destroyed by OLD are irreparable by T4 RNA ligase. • Both cuts occur after the first cytosine within a paired CNG pseudo-palindrome.
• A phage-inspired genetic screen to identify the targets of tRNA nucleases.
• OLD severs the anticodon stem with a one-base staggered cut.
• tRNAs destroyed by OLD are irreparable by T4 RNA ligase. • Both cuts occur after the first cytosine within a paired CNG pseudo-palindrome.