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Mass spectrometry of the serum coating revealed key ECM proteins like vitronectin, fibronectin, and collagens. These proteins likely play a crucial role in supporting naive hPSC culture and differentiation.

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Plus, exome sequencing revealed fewer DNA mutations compared to MEF cultures. 🧬

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Blastoids generated from multiple naive hPSC lines - after expansion on serum - formed both embryonic and extraembryonic cell lineages.

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and also high blastoid formation efficiency

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Naive hPSCs on serum retained high trophectoderm potential

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Naive hPSCs on serum coatings also showed efficient developmental progression via capacitation and germ layer specification

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Serum coating eliminated fibroblast contamination from RNA sequencing analyses. 🧹✅

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Here’s what we found:
Growth rate, clonogenicity, and global gene expression profile on serum coating were comparable to MEF-based cultures.

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Yes! We adapted naive hPSCs (both induced and embryonic origins) to a feeder-free, serum-based coating in PXGL medium. 🧫
This method worked across 8 hPSC lines in 5 labs, showing robust growth for more than 20 passages! 💪

With pleasure we share our preprint reporting robust and cost-effective expansion of naive human Pluripotent Stem Cells without feeder cells. A fantastic collaborative effort with @leeboratory.bsky.social @jzylicz.bsky.social @pasquelab.bsky.social and Nicolas Rivron.

tinyurl.com/5n6z9rt9