For therapeutics, we implemented a UV-damage kreatinocyte model and see if the supernatant of an engineered C. acnes producing a recombinant antioxidant protein could protect the cells. Turns out it reduced significantly the ROS levels in the UV exposed keratinocytes!

For biocontainment, we focused on targeting key metabolic genes so C. acnes would only survive if we topically supplement the missing amino acid. Since knocking out genes is still challenging in this skin non-model bacterium, we turned to #CRISPRi to quickly screen auxotrophs.

Thus, we have focused in engineering C. acnes. Now, we offer a whole set of tools for making C. acnes smarter with:
🔨 Modular and optimized plasmid
🔨 Basic expression tools: promoters, RBSs, terminators…
🔨 CRISPRi
🔨 Natural and synthetic inducible systems