David Gilot
@gilotd.bsky.social
Experienced mechanistic biology and drug discovery professional. Innovative therapies. Personal views.
Reposted by David Gilot
Can amylin-based drugs upstage GLP-1 agonists? They could avoid muscle loss and gastrointestinal side effects of current obesity therapies. My latest for @natbiotech.nature.com rdcu.be/ezwbM
Can amylin-based drugs upstage GLP-1 agonists?
Nature Biotechnology - Analogs of the pancreatic peptide hormone amylin are making strides in obesity as developers zoom in on oral drugs that can emulate or even improve on injectable GLP-1...
rdcu.be
August 6, 2025 at 1:09 PM
Can amylin-based drugs upstage GLP-1 agonists? They could avoid muscle loss and gastrointestinal side effects of current obesity therapies. My latest for @natbiotech.nature.com rdcu.be/ezwbM
FDA Commissioner Martin A. Makary :“By leveraging AI-based computational modeling, human organ model-based lab testing, and real-world human data, we can get safer treatments to patients faster and more reliably, while also reducing R&D costs and drug prices. It is a win-win for public health …”
FDA Announces Plan to Phase Out Animal Testing Requirement for Monoclonal Antibodies and Other Drugs
FDA Plans to Phase Out Animal Testing Requirement
www.fda.gov
April 11, 2025 at 4:26 AM
FDA Commissioner Martin A. Makary :“By leveraging AI-based computational modeling, human organ model-based lab testing, and real-world human data, we can get safer treatments to patients faster and more reliably, while also reducing R&D costs and drug prices. It is a win-win for public health …”
www.science.org/doi/10.1126/...
One more time; one protein - 2 functions. Really nice story. miRNA.
One more time; one protein - 2 functions. Really nice story. miRNA.
Noncanonical role of ALAS1 as a heme-independent inhibitor of small RNA–mediated silencing
microRNAs (miRNAs) and small interfering RNAs (siRNAs) are 21- to 22-nucleotide RNAs that guide Argonaute-class effectors to targets for repression. In this work, we uncover 5-aminolevulinic acid synt...
www.science.org
December 20, 2024 at 6:02 PM
www.science.org/doi/10.1126/...
One more time; one protein - 2 functions. Really nice story. miRNA.
One more time; one protein - 2 functions. Really nice story. miRNA.
www.biorxiv.org/content/10.1...
Ago2 activity : ‘Expansion of the guide–target duplex major groove docks the scissile phosphate for hydrolysis and subsequent groove compression after position 16 permits target RNAs to exit the catalytic cleft. ´
Ago2 activity : ‘Expansion of the guide–target duplex major groove docks the scissile phosphate for hydrolysis and subsequent groove compression after position 16 permits target RNAs to exit the catalytic cleft. ´
Structural basis for gene silencing by siRNAs in humans
Small interfering RNAs (siRNAs) guide mRNA cleavage by human Argonaute2 (hAgo2), leading to targeted gene silencing. Despite their laboratory and clinical impact, structural insights into human siRNA ...
www.biorxiv.org
December 7, 2024 at 8:58 AM
www.biorxiv.org/content/10.1...
Ago2 activity : ‘Expansion of the guide–target duplex major groove docks the scissile phosphate for hydrolysis and subsequent groove compression after position 16 permits target RNAs to exit the catalytic cleft. ´
Ago2 activity : ‘Expansion of the guide–target duplex major groove docks the scissile phosphate for hydrolysis and subsequent groove compression after position 16 permits target RNAs to exit the catalytic cleft. ´
www.biorxiv.org/content/10.1...
Tour de force : cell painting - phenotypic impacts of perturbing 15,243 human genes, including overexpressing 12,609 genes (using open reading frames, ORFs) and knocking out 7,975 genes (using CRISPR-Cas9) in U2OS cells.
Tour de force : cell painting - phenotypic impacts of perturbing 15,243 human genes, including overexpressing 12,609 genes (using open reading frames, ORFs) and knocking out 7,975 genes (using CRISPR-Cas9) in U2OS cells.
Morphological map of under- and over-expression of genes in human cells
Cell Painting images offer valuable insights into the state of a cell and enable many biological applications, but publicly available arrayed datasets only include hundreds of genes perturbed. The JUM...
www.biorxiv.org
December 6, 2024 at 5:02 PM
www.biorxiv.org/content/10.1...
Tour de force : cell painting - phenotypic impacts of perturbing 15,243 human genes, including overexpressing 12,609 genes (using open reading frames, ORFs) and knocking out 7,975 genes (using CRISPR-Cas9) in U2OS cells.
Tour de force : cell painting - phenotypic impacts of perturbing 15,243 human genes, including overexpressing 12,609 genes (using open reading frames, ORFs) and knocking out 7,975 genes (using CRISPR-Cas9) in U2OS cells.