Gerry Hammond
@gerryhammond.bsky.social
Basic. Cell Biologist. Lipid signaling and phosphoinositide enthusiast.
Announcing our new PA biosensor, PILS-Nir1 – seen here in HeLa cells during PLC activation #lipidtime #phospholipids
Read the OA paper in JCB
Read the OA paper in JCB
September 10, 2025 at 7:56 PM
Announcing our new PA biosensor, PILS-Nir1 – seen here in HeLa cells during PLC activation #lipidtime #phospholipids
Read the OA paper in JCB
Read the OA paper in JCB
They’re lining up to learn more after @wumin-lab.bsky.social gave a fantastic talk on dynamic PI signaling in the opening #lipids and membranes session at #asbmb25!
April 13, 2025 at 4:31 PM
They’re lining up to learn more after @wumin-lab.bsky.social gave a fantastic talk on dynamic PI signaling in the opening #lipids and membranes session at #asbmb25!
Emma Drew from U Oregon giving an amazing talk in the first #lipids and membranes session at #ASBMB2025. Single molecule experiments to show real time activation of a lipid phosphatase 🤯
April 13, 2025 at 3:08 PM
Emma Drew from U Oregon giving an amazing talk in the first #lipids and membranes session at #ASBMB2025. Single molecule experiments to show real time activation of a lipid phosphatase 🤯
April 13, 2025 at 2:26 PM
As an added bonus, the quantitative data reveals superior properties of these single-molecule-level biosensors:
February 11, 2025 at 4:03 PM
As an added bonus, the quantitative data reveals superior properties of these single-molecule-level biosensors:
We quantified it here:
February 11, 2025 at 4:03 PM
We quantified it here:
Since this occurs because the biosensor is outcompeting the native AKT1 molecules, Victoria tried expressing biosensors at lower levels where we resolve single biosensor molecules (shown in magenta). Hey presto! No inhibition:
February 11, 2025 at 4:03 PM
Since this occurs because the biosensor is outcompeting the native AKT1 molecules, Victoria tried expressing biosensors at lower levels where we resolve single biosensor molecules (shown in magenta). Hey presto! No inhibition:
This happens with all of the PIP3 biosensors we tested, even the low-affinity ones:
February 11, 2025 at 4:03 PM
This happens with all of the PIP3 biosensors we tested, even the low-affinity ones:
Now Victoria expressed the most popular PIP3 biosensor - the PH domain from the very same AKT1 protein (pink). The biosensor recruits very nicely with EGF, reporting PIP3 increases - but completely kills native AKT1 recruitment, as you see here:
February 11, 2025 at 4:03 PM
Now Victoria expressed the most popular PIP3 biosensor - the PH domain from the very same AKT1 protein (pink). The biosensor recruits very nicely with EGF, reporting PIP3 increases - but completely kills native AKT1 recruitment, as you see here:
Morgan tagged endogenous AKT1 with NeonGreen. Expression is low enough that you see single molecules at the cell surface in TIRF, which are recruited by PIP3 when the cell is stimulated with EGF:
February 11, 2025 at 4:03 PM
Morgan tagged endogenous AKT1 with NeonGreen. Expression is low enough that you see single molecules at the cell surface in TIRF, which are recruited by PIP3 when the cell is stimulated with EGF:
To everyone that celebrates it…
December 24, 2024 at 8:53 PM
To everyone that celebrates it…
#proudmentor! Great @FASEBorg #phospholipids meeting in MN with Morgan Ricci picking up best poster and Claire Weckerly getting a best short talk! This is why I don’t meddle in my trainees’ presentations…
July 30, 2024 at 4:09 PM
#proudmentor! Great @FASEBorg #phospholipids meeting in MN with Morgan Ricci picking up best poster and Claire Weckerly getting a best short talk! This is why I don’t meddle in my trainees’ presentations…
Happy fourth to all you scientists who celebrate it!
July 4, 2024 at 5:16 PM
Happy fourth to all you scientists who celebrate it!
My lab having a fight about this: #lipidtime enthusiasts, did they pick the right PIP2?
April 10, 2024 at 4:22 PM
My lab having a fight about this: #lipidtime enthusiasts, did they pick the right PIP2?