GERG
@gerg77.bsky.social
BS RIT | PhD UMich - Janet Smith Lab | Postdoc MIT - Imperiali & Kiessling Labs |
Ruth L. Kirschstein Fellow | Senior Research Fellow @ Biogen
Membrane proteins, glycobiology, structural biology, bikes, dogs, bills mafia
Ruth L. Kirschstein Fellow | Senior Research Fellow @ Biogen
Membrane proteins, glycobiology, structural biology, bikes, dogs, bills mafia
July 5, 2025 at 10:07 PM
June 19, 2025 at 3:38 PM
June 10, 2025 at 11:52 PM
June 6, 2025 at 4:37 PM
June 6, 2025 at 2:14 PM
But here's the real beauty - the binding curves! Our selected nanobodies show high affinity binding with KD values from 15-200 nM. 🎯
May 25, 2025 at 6:32 PM
But here's the real beauty - the binding curves! Our selected nanobodies show high affinity binding with KD values from 15-200 nM. 🎯
The magic happens during selection! Look at this beautiful enrichment of WbaP binders over successive rounds:
From 0.7% → 18% positive binders! Even protein concentrations as low as 100 nM
From 0.7% → 18% positive binders! Even protein concentrations as low as 100 nM
May 25, 2025 at 6:32 PM
The magic happens during selection! Look at this beautiful enrichment of WbaP binders over successive rounds:
From 0.7% → 18% positive binders! Even protein concentrations as low as 100 nM
From 0.7% → 18% positive binders! Even protein concentrations as low as 100 nM
Here's our selection strategy! We use the same dual-Strep tag for both purification AND magnetic bead capture. No protein modification needed!
The workflow: Negative sorts → Positive selection → Amplify → Repeat 🔄
The workflow: Negative sorts → Positive selection → Amplify → Repeat 🔄
May 25, 2025 at 6:32 PM
Here's our selection strategy! We use the same dual-Strep tag for both purification AND magnetic bead capture. No protein modification needed!
The workflow: Negative sorts → Positive selection → Amplify → Repeat 🔄
The workflow: Negative sorts → Positive selection → Amplify → Repeat 🔄
Whelp looks like I'm sleeping on the couch now
April 18, 2025 at 1:13 AM
Whelp looks like I'm sleeping on the couch now
March 28, 2025 at 11:28 PM
March 15, 2025 at 11:50 PM
March 14, 2025 at 10:59 PM
January 26, 2025 at 11:16 PM
January 20, 2025 at 3:56 AM
January 20, 2025 at 1:02 AM
Holiday cooking class for work!
December 4, 2024 at 6:05 PM
Holiday cooking class for work!