You could also use a typewriter to type your answer and ask the reviewer if he recommends the use of home-casted gels to perform the western blots.

Here is a good way to waste $$$ but turn a reviewer a mass spec believer. Buy 3 antibodies: the first well validated, and 2 from « less reputable » suppliers. Then show that WB gives you discrepant results. Now point to the reviewer that antibodies are not more reliable than mass spectrometry.

4/4 The team identified key EMC3 interacting proteins, including VCP, and demonstrated how the VCP inhibitor CB5083 restores alveolar structure and trafficking in AT2 patient-specific iPSCs 💊.

3/4 Proteomics revealed that Emc3 depletion fixes vesicle trafficking and mitochondrial dysfunction linked to the mutation. This could be a game-changer for treatment! 🧫

2/4 In this groundbreaking work by an incredible collaborative team, we used a mouse model to show how the deletion of Emc3 in AT2 cells can rescue defects caused by the I73T mutation. 🐭🔬