Jan Gawor
@gaworjan.bsky.social
Sanger / NGS sequencing specialist (love long reads :) / microbial de novo sequencing / Research Associate at http://oligo.pl DNA Sequencing Facility IBB PAS Warsaw, Poland
Please check this on agarose gel and confirm the concentration by densitometry. Typically such high OD is due to the remaining RNA in miniprep.
August 7, 2025 at 7:13 AM
Please check this on agarose gel and confirm the concentration by densitometry. Typically such high OD is due to the remaining RNA in miniprep.
There is also briliant paper regarding the complexity in bacterial genomes:
academic.oup.com/nar/article/...
academic.oup.com/nar/article/...
Pushing the limits of de novo genome assembly for complex prokaryotic genomes harboring very long, near identical repeats
Abstract. Generating a complete, de novo genome assembly for prokaryotes is often considered a solved problem. However, we here show that Pseudomonas koree
academic.oup.com
May 15, 2025 at 9:50 PM
There is also briliant paper regarding the complexity in bacterial genomes:
academic.oup.com/nar/article/...
academic.oup.com/nar/article/...