Matt Gartner
@gartnerm94.bsky.social
Virologist 🦠 at the Doherty Institute.
It’s certainly possible. We’ve tried to grow our clinical OC43 viruses in a range of cell lines with no success. We haven’t tried organoids from different tissues.
September 11, 2025 at 1:15 PM
It’s certainly possible. We’ve tried to grow our clinical OC43 viruses in a range of cell lines with no success. We haven’t tried organoids from different tissues.
Our findings show that contemporary viruses should be used for the study of seasonal coronavirus biology and evaluation of medical countermeasures.
August 27, 2025 at 11:49 PM
Our findings show that contemporary viruses should be used for the study of seasonal coronavirus biology and evaluation of medical countermeasures.
We also compared the replication of isolates to the lab strain in models of the human respiratory tract and found differences in respiratory tract tropism.
August 27, 2025 at 11:49 PM
We also compared the replication of isolates to the lab strain in models of the human respiratory tract and found differences in respiratory tract tropism.
We made stocks of these isolates in immortalised cell lines. NL63 isolates replicated in LLC-MK2 cells and this was boosted by ACE2 and TMPRSS2 over-expression. 229E isolates replicated well in Huh7 cells, with a modest increase in the isolates when we over-expressed TMPRSS2.
August 27, 2025 at 11:49 PM
We made stocks of these isolates in immortalised cell lines. NL63 isolates replicated in LLC-MK2 cells and this was boosted by ACE2 and TMPRSS2 over-expression. 229E isolates replicated well in Huh7 cells, with a modest increase in the isolates when we over-expressed TMPRSS2.
We found these isolates showed the highest genetic variability in their Spikes compared to lab-adapted reference strains.
August 27, 2025 at 11:49 PM
We found these isolates showed the highest genetic variability in their Spikes compared to lab-adapted reference strains.
We used human airway and alveolar epithelial cultures to recover 15 contemporary isolates of human coronaviruses NL63, 229E and OC43.
August 27, 2025 at 11:49 PM
We used human airway and alveolar epithelial cultures to recover 15 contemporary isolates of human coronaviruses NL63, 229E and OC43.
Most studies focusing on seasonal coronaviruses are limited to lab-adapted strains that were isolated decades ago and have been passaged in cell culture who knows how many times.
August 27, 2025 at 11:49 PM
Most studies focusing on seasonal coronaviruses are limited to lab-adapted strains that were isolated decades ago and have been passaged in cell culture who knows how many times.