Dr Gaétan Burgio.
@gaetanburgio.bsky.social
Group leader, Australian National University (ANU), Australia. Interested in microbial infections & CRISPR gene editing. Opinions and views are my own. https://jcsmr.anu.edu.au/research/groups/burgio-group-genome-editing-and-microbial-immunity
We next wondered how the NUC/gate are the driver of these differences between the Cas12a orthologues? To answer this question the Palermo lab performed series of molecular dynamics simulations. They showed that the difference in flexibility in this region between the orthologues is the driver.
March 30, 2025 at 10:52 AM
We next wondered how the NUC/gate are the driver of these differences between the Cas12a orthologues? To answer this question the Palermo lab performed series of molecular dynamics simulations. They showed that the difference in flexibility in this region between the orthologues is the driver.
On the target trajectory, we looked at an important region called the NUC loop that is extended in AsCas12a but not other orthologues. We wondered wether this region is important. Short answer is yes. by mutating this region, it kills all activities.
March 30, 2025 at 10:48 AM
On the target trajectory, we looked at an important region called the NUC loop that is extended in AsCas12a but not other orthologues. We wondered wether this region is important. Short answer is yes. by mutating this region, it kills all activities.