Florent Waltz
@florentwaltz.bsky.social
SNSF Ambizione Project Leader
Looking at tiny frozen mitochondria with big microscopes 🔬 cryoEM/cryoET
Green organisms 🌿 & evolution 🦠
Biozentrum, hosted in the Engel Lab, CH
🇫🇷 -> 🇩🇪 -> 🇨🇭
Website: https://www.waltzresearch.com/
Looking at tiny frozen mitochondria with big microscopes 🔬 cryoEM/cryoET
Green organisms 🌿 & evolution 🦠
Biozentrum, hosted in the Engel Lab, CH
🇫🇷 -> 🇩🇪 -> 🇨🇭
Website: https://www.waltzresearch.com/
Last Friday (17th), we had an amazing first Young Swiss Tomo meeting in Bern, organized with Elda Bauda, Zanetta Kechagia, and @leonieanton.bsky.social!
Over 70 people from 🇨🇭 gathered to talk about cryo-electron tomography 🔬.
Huge thanks to the DCI for making the event possible! See you next year! 👋
Over 70 people from 🇨🇭 gathered to talk about cryo-electron tomography 🔬.
Huge thanks to the DCI for making the event possible! See you next year! 👋
October 19, 2025 at 9:16 AM
Last Friday (17th), we had an amazing first Young Swiss Tomo meeting in Bern, organized with Elda Bauda, Zanetta Kechagia, and @leonieanton.bsky.social!
Over 70 people from 🇨🇭 gathered to talk about cryo-electron tomography 🔬.
Huge thanks to the DCI for making the event possible! See you next year! 👋
Over 70 people from 🇨🇭 gathered to talk about cryo-electron tomography 🔬.
Huge thanks to the DCI for making the event possible! See you next year! 👋
I had a wonderful time in Japan 🗼! Huge thanks to @ken-wakaba.bsky.social for inviting me to Sangyo University in Kyoto. Thanks to Takashi Yamano (and Ken-ichi) for organizing the Beyond the Green 🍀 session at the Japanese Botanical Society meeting!
Great science and discussions!
Great science and discussions!
September 22, 2025 at 2:59 AM
I had a wonderful time in Japan 🗼! Huge thanks to @ken-wakaba.bsky.social for inviting me to Sangyo University in Kyoto. Thanks to Takashi Yamano (and Ken-ichi) for organizing the Beyond the Green 🍀 session at the Japanese Botanical Society meeting!
Great science and discussions!
Great science and discussions!
Re-redeading this one 📖. Great book for evolution and bioenergetics aficionados. The view is a bit distracting though ⛵
May 19, 2025 at 1:28 PM
Re-redeading this one 📖. Great book for evolution and bioenergetics aficionados. The view is a bit distracting though ⛵
Ribosomes for everyone
May 7, 2025 at 11:02 AM
Ribosomes for everyone
You want to start tomography? Solve structures inside cells? Reach Nyquist 😳 ? @phaips.vd.st and I have a website for you! tomoguide.github.io
You'll find a tutorial on how to reconstruct tomograms, pick particles and do subtomogram averaging, using different software!
Hope it will be useful !
You'll find a tutorial on how to reconstruct tomograms, pick particles and do subtomogram averaging, using different software!
Hope it will be useful !
May 6, 2025 at 4:42 PM
You want to start tomography? Solve structures inside cells? Reach Nyquist 😳 ? @phaips.vd.st and I have a website for you! tomoguide.github.io
You'll find a tutorial on how to reconstruct tomograms, pick particles and do subtomogram averaging, using different software!
Hope it will be useful !
You'll find a tutorial on how to reconstruct tomograms, pick particles and do subtomogram averaging, using different software!
Hope it will be useful !
Finally, we measured the enzymatic activity of our complexes, comparing in situ and purified samples, revealing a correlation between their activity and structural conformation—particularly in the case of complex I.
This was done by Thalia :)
This was done by Thalia :)
March 21, 2025 at 2:09 PM
Finally, we measured the enzymatic activity of our complexes, comparing in situ and purified samples, revealing a correlation between their activity and structural conformation—particularly in the case of complex I.
This was done by Thalia :)
This was done by Thalia :)
We added a nice comparison of the different supercomplexes observed to date in different organisms
March 21, 2025 at 2:09 PM
We added a nice comparison of the different supercomplexes observed to date in different organisms
We also showed that the few respirasomes that we found at the inner boundary membrane have the same overall architecture as the ones in cristae.
March 21, 2025 at 2:09 PM
We also showed that the few respirasomes that we found at the inner boundary membrane have the same overall architecture as the ones in cristae.
A lot of the new cool things are hidden in the supp. 🥷
First, we added several metrics regarding the concentration and organization of respirasomes within the cristae, as well as their influence on membrane curvature.
This was done using Membrain by @lorenzlamm.bsky.social 💻
First, we added several metrics regarding the concentration and organization of respirasomes within the cristae, as well as their influence on membrane curvature.
This was done using Membrain by @lorenzlamm.bsky.social 💻
March 21, 2025 at 2:09 PM
A lot of the new cool things are hidden in the supp. 🥷
First, we added several metrics regarding the concentration and organization of respirasomes within the cristae, as well as their influence on membrane curvature.
This was done using Membrain by @lorenzlamm.bsky.social 💻
First, we added several metrics regarding the concentration and organization of respirasomes within the cristae, as well as their influence on membrane curvature.
This was done using Membrain by @lorenzlamm.bsky.social 💻
I'm super happy that our story is now published!
📖 www.science.org/doi/10.1126/...
But what changed compared to the original preprint?
Also, I feel i should post Movie 1 🎥, that inspired the cover. Back when I did the original bluesky thread, movies were not available.
📖 www.science.org/doi/10.1126/...
But what changed compared to the original preprint?
Also, I feel i should post Movie 1 🎥, that inspired the cover. Back when I did the original bluesky thread, movies were not available.
March 21, 2025 at 2:09 PM
I'm super happy that our story is now published!
📖 www.science.org/doi/10.1126/...
But what changed compared to the original preprint?
Also, I feel i should post Movie 1 🎥, that inspired the cover. Back when I did the original bluesky thread, movies were not available.
📖 www.science.org/doi/10.1126/...
But what changed compared to the original preprint?
Also, I feel i should post Movie 1 🎥, that inspired the cover. Back when I did the original bluesky thread, movies were not available.
Not the straightest, but we'll make it work 🟥♦️🟥♦️
March 19, 2025 at 6:42 PM
Not the straightest, but we'll make it work 🟥♦️🟥♦️
Wooooooooo (lift in)
March 19, 2025 at 6:42 PM
Wooooooooo (lift in)
Weeeeeeeee (lift out)
March 19, 2025 at 6:42 PM
Weeeeeeeee (lift out)
Running some experiments in Lausanne. The view never disappoints
March 18, 2025 at 12:40 PM
Running some experiments in Lausanne. The view never disappoints
We (@cellarchlab.com peeps, @manondemulder.bsky.social @phaips.vd.st @fannyleblanc.bsky.social @computingcaitie.bsky.social and Ricardo) had a blast at the EMBO in situ meeting in Heidelberg! Meeting old friends and making new ones! Lots of cool science and new ideas to try 👨🏻🔬🔬
February 6, 2025 at 7:27 PM
We (@cellarchlab.com peeps, @manondemulder.bsky.social @phaips.vd.st @fannyleblanc.bsky.social @computingcaitie.bsky.social and Ricardo) had a blast at the EMBO in situ meeting in Heidelberg! Meeting old friends and making new ones! Lots of cool science and new ideas to try 👨🏻🔬🔬
A bit late, but congratulations to my colleague @wojwie.bsky.social for this one 🎉
My fav. part is Fig. 8, it clearly illustrates why you want to look at things inside cells 🔬
The difference between in situ and "in situ". You never know what happens in broken organelles 👀
📖https://shorturl.at/YxwPE
My fav. part is Fig. 8, it clearly illustrates why you want to look at things inside cells 🔬
The difference between in situ and "in situ". You never know what happens in broken organelles 👀
📖https://shorturl.at/YxwPE
November 28, 2024 at 12:10 PM
A bit late, but congratulations to my colleague @wojwie.bsky.social for this one 🎉
My fav. part is Fig. 8, it clearly illustrates why you want to look at things inside cells 🔬
The difference between in situ and "in situ". You never know what happens in broken organelles 👀
📖https://shorturl.at/YxwPE
My fav. part is Fig. 8, it clearly illustrates why you want to look at things inside cells 🔬
The difference between in situ and "in situ". You never know what happens in broken organelles 👀
📖https://shorturl.at/YxwPE
Working in real life cryo conditions
November 21, 2024 at 5:34 PM
Working in real life cryo conditions
November 11, 2024 at 11:59 AM
November 11, 2024 at 11:59 AM
Finally got some time to properly test #RELION5 complete pipeline, starting from raw frames to STA. 22 tilt-series (from EMPIAR-11830), picking done with pytom, 14600 final particles, FSC 4.1-ish on the LSU (Nyquist bin1 3.8). Ofc it's a ribosome 😅 but now I can try with smthng more chlengin
#CryoET
#CryoET
November 10, 2024 at 7:11 PM
Check out our new preprint on plant🌱mitoribosome!
We captured it in action, translating mRNA, and combining cryo-EM 🔬 and Nanopore sequencing 🧬, identified key rRNA modifications. Plus we delve into a late-stage maturation step of the small subunit.
📖: www.biorxiv.org/content/10.1...
#CryoEM
We captured it in action, translating mRNA, and combining cryo-EM 🔬 and Nanopore sequencing 🧬, identified key rRNA modifications. Plus we delve into a late-stage maturation step of the small subunit.
📖: www.biorxiv.org/content/10.1...
#CryoEM
October 30, 2024 at 7:54 PM
Check out our new preprint on plant🌱mitoribosome!
We captured it in action, translating mRNA, and combining cryo-EM 🔬 and Nanopore sequencing 🧬, identified key rRNA modifications. Plus we delve into a late-stage maturation step of the small subunit.
📖: www.biorxiv.org/content/10.1...
#CryoEM
We captured it in action, translating mRNA, and combining cryo-EM 🔬 and Nanopore sequencing 🧬, identified key rRNA modifications. Plus we delve into a late-stage maturation step of the small subunit.
📖: www.biorxiv.org/content/10.1...
#CryoEM
We also saw many other cool things 👀, like the native organization of ATP synthases, in stacked rows of dimers, inducing the membrane curvature at the the cristae tips, or circular ⭕ mitoribosome polysomes at the inner membrane. So much to see !
September 5, 2024 at 7:54 AM
We also saw many other cool things 👀, like the native organization of ATP synthases, in stacked rows of dimers, inducing the membrane curvature at the the cristae tips, or circular ⭕ mitoribosome polysomes at the inner membrane. So much to see !
This suggests that cyt. c are probably freely diffusing in the IMS between respirasomes, supporting the "free diffusion model" rather than the "substrate channeling model" where a specific pool of cyt. c and ubiquinone would only shuttle within respirasomes and not between them
September 5, 2024 at 7:53 AM
This suggests that cyt. c are probably freely diffusing in the IMS between respirasomes, supporting the "free diffusion model" rather than the "substrate channeling model" where a specific pool of cyt. c and ubiquinone would only shuttle within respirasomes and not between them
From the subtome average we also resolved cyt. c densities bound to the respirasame. Interestingly, the distances from complex III te complex IV binding sites range from ~90 Å to ~230 Å with an average distance of ~175 Å, which is quite far!
September 5, 2024 at 7:52 AM
From the subtome average we also resolved cyt. c densities bound to the respirasame. Interestingly, the distances from complex III te complex IV binding sites range from ~90 Å to ~230 Å with an average distance of ~175 Å, which is quite far!