Tutorial using example data and example analysis pipeline has been also updated. github.com/astanabe/Cla...

使い捨てプラカップと使い捨てロープを使用するようになると、バケツやロープの漂白が不要になるため、大変手間が減って楽になります。また、外壁塗装に使われるアルミローラーポールの先端にロープを結べば、柄杓代わりにもなります。風が強くて水面に落とせないときは、精製水を入れて重くします。

In the latest version, installer for macOS has been fixed. A bug of coverage-based rarefaction of ASV-saturated samples in clrarefysum has been fixed. A bug of outputmultihit option of clsplitseq has been fixed. The bundled databases have not been updated.

In addition, decontamination based on DNA concentration estimated using concentration-known internal standard DNA has been supported in "clremovecontam" command.

In the latest Claident, "clrarefysum" command has been added. By this command, coverage-based rarefaction can be performed quickly.

And Japanese manual can be found at the following.
github.com/astanabe/eDN...

Example analysis scripts are available at the following.
github.com/astanabe/Cla...