Fang Dong-CFPS
@fang-dong.bsky.social
biochemistry, biophysics & electrophysiology. currently learning scientific illustration/blender. I am good at protein production without cells
This strategy is also useful for producing GPCRs, RTKs, or membrane-bound antigen–antibody complexes — whether for structure determination, mechanistic insights, or high-throughput assays.
▶️ Thanks for watching!
▶️ Thanks for watching!
August 26, 2025 at 9:12 AM
This strategy is also useful for producing GPCRs, RTKs, or membrane-bound antigen–antibody complexes — whether for structure determination, mechanistic insights, or high-throughput assays.
▶️ Thanks for watching!
▶️ Thanks for watching!
• Microsomal xpression yield up to 0.1 mg/mL
• Amphipathic copolymers extract the complete transporter–binder–substrate complex, assembling into native nanodiscs (Cube Biotech) in a detergent-free manner for structural studies
Representive structure in video is PIN1 with Sybody PDB 7Y9V.
• Amphipathic copolymers extract the complete transporter–binder–substrate complex, assembling into native nanodiscs (Cube Biotech) in a detergent-free manner for structural studies
Representive structure in video is PIN1 with Sybody PDB 7Y9V.
August 26, 2025 at 9:11 AM
• Microsomal xpression yield up to 0.1 mg/mL
• Amphipathic copolymers extract the complete transporter–binder–substrate complex, assembling into native nanodiscs (Cube Biotech) in a detergent-free manner for structural studies
Representive structure in video is PIN1 with Sybody PDB 7Y9V.
• Amphipathic copolymers extract the complete transporter–binder–substrate complex, assembling into native nanodiscs (Cube Biotech) in a detergent-free manner for structural studies
Representive structure in video is PIN1 with Sybody PDB 7Y9V.
In this concept:
• A signal peptide directs the translated transporter into microsomal lumen in an inside-out orientation
• Cytosolic loops are stabilized by co-expressed synthetic nanobodies (screening also by CFPS)
• Auxin substrate is added to enhance folding efficiency during translation
• A signal peptide directs the translated transporter into microsomal lumen in an inside-out orientation
• Cytosolic loops are stabilized by co-expressed synthetic nanobodies (screening also by CFPS)
• Auxin substrate is added to enhance folding efficiency during translation
August 26, 2025 at 9:10 AM
In this concept:
• A signal peptide directs the translated transporter into microsomal lumen in an inside-out orientation
• Cytosolic loops are stabilized by co-expressed synthetic nanobodies (screening also by CFPS)
• Auxin substrate is added to enhance folding efficiency during translation
• A signal peptide directs the translated transporter into microsomal lumen in an inside-out orientation
• Cytosolic loops are stabilized by co-expressed synthetic nanobodies (screening also by CFPS)
• Auxin substrate is added to enhance folding efficiency during translation
ALiCE® preserves the complete eukaryotic transcription and translation machinery of plant cells and contains microsomes: ER-derived lipid vesicles where PIN-FORMED auxin exporter- PIN5, PIN6, PIN8, and PILS are natively localized. @bjornppedersen.bsky.social Would you consider cell-free worth a try?
August 26, 2025 at 9:09 AM
ALiCE® preserves the complete eukaryotic transcription and translation machinery of plant cells and contains microsomes: ER-derived lipid vesicles where PIN-FORMED auxin exporter- PIN5, PIN6, PIN8, and PILS are natively localized. @bjornppedersen.bsky.social Would you consider cell-free worth a try?
aromatic residue, probably Phenylalanine？
August 18, 2025 at 5:27 PM
aromatic residue, probably Phenylalanine？
Skeuomorphism of GPCR signaling pathway (cycles rendering). High res video: youtu.be/Pfpjt0apBX4?...
August 18, 2025 at 10:02 AM
Skeuomorphism of GPCR signaling pathway (cycles rendering). High res video: youtu.be/Pfpjt0apBX4?...
🎥 This animation visualizes the pipeline of cell-free produced H2R receptor, as published by the Bernhard group (PDB: 8POK). Frank Bernhard, Zoe Köck. Reference: Nature Communications, DOI: 10.1038/s41467-024-46096-z
The video was rendered by Blender 5.0
The video was rendered by Blender 5.0
August 18, 2025 at 9:37 AM
🎥 This animation visualizes the pipeline of cell-free produced H2R receptor, as published by the Bernhard group (PDB: 8POK). Frank Bernhard, Zoe Köck. Reference: Nature Communications, DOI: 10.1038/s41467-024-46096-z
The video was rendered by Blender 5.0
The video was rendered by Blender 5.0
• In addition to the ligand, the heterotrimeric G protein and nanobody help stabilize the receptor in a native-like conformation and preserve its functional state.
• Cryo-EM sample in 24h, 1-2 mL reaction sufficient for 8~10 grids
• Cryo-EM sample in 24h, 1-2 mL reaction sufficient for 8~10 grids
August 18, 2025 at 9:36 AM
• In addition to the ligand, the heterotrimeric G protein and nanobody help stabilize the receptor in a native-like conformation and preserve its functional state.
• Cryo-EM sample in 24h, 1-2 mL reaction sufficient for 8~10 grids
• Cryo-EM sample in 24h, 1-2 mL reaction sufficient for 8~10 grids
• To stabilize these highly dynamic membrane proteins, pre-assembled nanodiscs serve as a synthetic lipid bilayer system, mimicking the native membrane.
• Human Histamine Receptor H2R was co-translationally inserted into nanodiscs in a detergent-free manner.
• Human Histamine Receptor H2R was co-translationally inserted into nanodiscs in a detergent-free manner.
August 18, 2025 at 9:36 AM
• To stabilize these highly dynamic membrane proteins, pre-assembled nanodiscs serve as a synthetic lipid bilayer system, mimicking the native membrane.
• Human Histamine Receptor H2R was co-translationally inserted into nanodiscs in a detergent-free manner.
• Human Histamine Receptor H2R was co-translationally inserted into nanodiscs in a detergent-free manner.
Recent breakthroughs in solving the structures of human H2R and full-length β1AR using cell-free expression systems highlight the potential of CFPS as a broadly applicable strategy for structural biology.
August 18, 2025 at 9:36 AM
Recent breakthroughs in solving the structures of human H2R and full-length β1AR using cell-free expression systems highlight the potential of CFPS as a broadly applicable strategy for structural biology.