Thank you @narjournal.bsky.social and reviewers, and thank you team @hendersonlab.bsky.social @weinehue.bsky.social Brian forde and Matt Hemm, and Jack Bryant who was there from the beginning

I think there's a LOT more that can be done through transposon mutagenesis to query a genome through editing your transposon!

Like all high throughout screens we got some unusual results too, the biology of which is still to be explored ('nested' genes??)

Some of these small genes are conserved and/or neighbouring well chracterised genes. Highlights that small genes/proteins are still overlooked.

Clearly more unannotated coding potential within E. coli. Interesting to see what different methods do and don't identify.

We did this in E. coli and identified some new small proteins, validated by western blot.