We also show:
🔹 How to infect placental tissue with these pathogens
🔹 How to dissociate the tissue into a single-cell suspension
🔹 How to enrich immune cells for scRNA-seq analysis 🧬

This protocol provides reproducible framework to explore host-pathogen interactions at single-cell resolution 🔥

This protocol details how to culture three key pathogens🦠:
• Listeria monocytogenes
• Plasmodium falciparum
• Toxoplasma gondii

And crucially — how to set up a placental ex vivo model that preserves cell viability and architecture. 🧪