These findings help to establish THG microscopy as a quantitative, multiscale imaging technique, paving the way for new applications. 📖 #Neuroscience #Myelin #THG #Microscopy #Research

Finally, we illustrate the relevance of pTHG microscopy for characterizing myelin distribution at different scales in fixed mouse and human brain tissue.

Fun fact for multiphoton microscopy geeks: aberrations caused by vertical micro-interfaces appear to be a significant contrast mechanism in polarized THG imaging. 😎

We show that axons exhibit complex pTHG profiles that depend on axon morphology and can't be explained by classical models. We establish a numerical strategy to relate pTHG profiles to axon diameter and myelin thickness, taking into account myelin-induced optical aberrations.

Our study presents a systematic characterization of polarized THG (pTHG) signals from individual axons, enabling the derivation of myelin scores in various systems. 🐭🐟
We confirm the sensitivity of THG for myelin in mouse tissue and detect early axon development in zebrafish.

By #J_Morizet #N_Olivier #MS_Aigrot #P_Mahou #E_Martin #A_Desmazieres #B_Stankoff @chiarastringari.bsky.social

🔬🌟 Find out how Third Harmonic Generation (THG) microscopy could help advance myelin biomedical research! 🧠

Un poste d’ingénieur en analyse d’images est ouvert en mobilité pour rejoindre @laboptbio.bsky.social à @polytechniqueparis.bsky.social (Palaiseau). Poste IR ouvert aux IE de la fonction publique et IR @cnrs.bsky.social. Deadline: 20/02. Lien (concours 508): profilsdemplois.cnrs.fr/ords/afipprd...

An image analysis engineer position is open for mobility to join @laboptbio.bsky.social at @polytechniqueparis.bsky.social ky.social in Palaiseau. IR position open to civil service IEs and CNRS IRs. Deadline: 20/02. Link (position #508): profilsdemplois.cnrs.fr/ords/afipprd...

Good analogy... It could also be viewed as: