Pratik Kumar
@dyechemist.bsky.social
Assitant Professor ncbsbangalore.bsky.social Making dye based molecular tools for imaging, cell biology and proteomics. Previously @hhmijanelia.bsky.social @stonybrooku.bsky.social, IISER-Kolkata, and JNCASR. Lab news: DyeCraftLab.com
Even Cooler: When BRD4 was translocated to constitutive heterochromatin, we observed increased transcriptional activity (histone H3.3 accumulation as a proxy). This shows that multifunctional fluorescent ligands can functionally alter chromatin in living cells!
January 28, 2025 at 5:17 AM
Even Cooler: When BRD4 was translocated to constitutive heterochromatin, we observed increased transcriptional activity (histone H3.3 accumulation as a proxy). This shows that multifunctional fluorescent ligands can functionally alter chromatin in living cells!
There’s More! Using the same Si-rhodamine dye, we created JQ1-containing fluorescent ligands to manipulate the localization of BRD4. @so-lets-kilab70.bsky.social showed rapid translocation of BRD4 from euchromatin to HaloTag sites at the nucleolus.
January 28, 2025 at 5:17 AM
There’s More! Using the same Si-rhodamine dye, we created JQ1-containing fluorescent ligands to manipulate the localization of BRD4. @so-lets-kilab70.bsky.social showed rapid translocation of BRD4 from euchromatin to HaloTag sites at the nucleolus.
These live-cell permeable biotins are great for affinity purification. Here, Jason Vevea figured out their in-cell labeling performance and purified mitochondria! This works so much better than the commercial biotin-HaloTag ligand (this doesn't work actually!)
January 28, 2025 at 5:17 AM
These live-cell permeable biotins are great for affinity purification. Here, Jason Vevea figured out their in-cell labeling performance and purified mitochondria! This works so much better than the commercial biotin-HaloTag ligand (this doesn't work actually!)
We could use this to guess apriori cell-impermeable (yellow) and cell-permeable (red and magenta) biotin-containing rhodamine ligands! We used imaging to verify this for HaloTag fusions at the cell surface, mitochondria, ER, and nucleus.
January 28, 2025 at 5:17 AM
We could use this to guess apriori cell-impermeable (yellow) and cell-permeable (red and magenta) biotin-containing rhodamine ligands! We used imaging to verify this for HaloTag fusions at the cell surface, mitochondria, ER, and nucleus.
🧵Prepint alert! Optimizing Multifunctional Fluorescent Ligands for Intracellular Labeling | tinyurl.com/3n55hvsc. With Jason Vevea, Ed Chapman, and @so-lets-kilab70.bsky.social, we combined dye chemistry, HaloTag, microscopy and cell biology to make protein purification and manipulation tools.
January 28, 2025 at 5:17 AM
🧵Prepint alert! Optimizing Multifunctional Fluorescent Ligands for Intracellular Labeling | tinyurl.com/3n55hvsc. With Jason Vevea, Ed Chapman, and @so-lets-kilab70.bsky.social, we combined dye chemistry, HaloTag, microscopy and cell biology to make protein purification and manipulation tools.
I will hire at all levels (postdoc, graduate students, JRF, interns). We will train you in chemical synthesis, spectroscopy, biological labelling and fluorescence imaging, among other things. We will strongly emphasize developing soft skills and anything relevant to your career.
January 7, 2025 at 7:16 PM
I will hire at all levels (postdoc, graduate students, JRF, interns). We will train you in chemical synthesis, spectroscopy, biological labelling and fluorescence imaging, among other things. We will strongly emphasize developing soft skills and anything relevant to your career.
We have a few projects in mind with existing collaborators, but I am open to brainstorming. Feel free to email me to discuss ideas or to say hi 😀😀. For more details on what I do, please visit pratik-kumar.com
January 7, 2025 at 7:16 PM
We have a few projects in mind with existing collaborators, but I am open to brainstorming. Feel free to email me to discuss ideas or to say hi 😀😀. For more details on what I do, please visit pratik-kumar.com
Super excited to start my lab @NCBS_Bangalore
in March 2025! The lab will primarily be powered by chemistry, and we will build chemical tools for biology with a strong focus on fluorescence imaging. We will collaborate widely and are open to any relevant areas of biology.
in March 2025! The lab will primarily be powered by chemistry, and we will build chemical tools for biology with a strong focus on fluorescence imaging. We will collaborate widely and are open to any relevant areas of biology.
January 7, 2025 at 7:16 PM
Super excited to start my lab @NCBS_Bangalore
in March 2025! The lab will primarily be powered by chemistry, and we will build chemical tools for biology with a strong focus on fluorescence imaging. We will collaborate widely and are open to any relevant areas of biology.
in March 2025! The lab will primarily be powered by chemistry, and we will build chemical tools for biology with a strong focus on fluorescence imaging. We will collaborate widely and are open to any relevant areas of biology.