Katy Hole
@drkhole.bsky.social
Postdoc @crick.ac.uk | Investigating astrocyte-neuron intercellular mitochondrial transfer | Views are my own | She/her
You're not wrong! There is some great work especially in the cancer field that uses updated tools to convincingly show transfer. However, the majority of astrocyte-neuron studies only use MitoTracker
July 11, 2025 at 4:24 PM
You're not wrong! There is some great work especially in the cancer field that uses updated tools to convincingly show transfer. However, the majority of astrocyte-neuron studies only use MitoTracker
13 of 16 papers used MitoTracker alone to identify transfer - we hope this work prompts a re-evaluation of the literature concerning astrocyte-neuron mitochondrial transfer
May 30, 2025 at 4:32 PM
13 of 16 papers used MitoTracker alone to identify transfer - we hope this work prompts a re-evaluation of the literature concerning astrocyte-neuron mitochondrial transfer
This builds on previous work showing that MitoTracker transfers without mitochondria in a contact-dependent manner. www.nature.com/articles/s42003-024-05964-6 Additionally, we find that MitoTracker can also transfer from astrocyte conditioned media (ACM) to neurons without mitochondria.
Exploring the limitations of mitochondrial dye as a genuine horizontal mitochondrial transfer surrogate - Communications Biology
Evidence underscores the non-specificity of mitochondrial dye in assessing horizontal mitochondrial transfer, suggesting that alternative approaches should be considered for gaining nuanced insights i...
www.nature.com
May 30, 2025 at 4:27 PM
This builds on previous work showing that MitoTracker transfers without mitochondria in a contact-dependent manner. www.nature.com/articles/s42003-024-05964-6 Additionally, we find that MitoTracker can also transfer from astrocyte conditioned media (ACM) to neurons without mitochondria.