Andi Wilson, PhD
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drandiwilson.bsky.social
Andi Wilson, PhD
@drandiwilson.bsky.social
NNF-funded postdoc at UCPH 🍄🦗🌿🇩🇰

I post about Science🔬 & Chronic Migraine 💊

She/Her 🏳️‍🌈

Opinions are my own
We hypothesize the expansions of:
1) HSP20 = good at surviving heat 🔥

2) GST = good at dealing with ROS generated during stress & in response to metabolism of PyOm ⚠️

3) ACD = good at metabolising PyOm 🍽

Taken together, R. undulata survives fire and then thrives in post-fire environments!
November 14, 2025 at 7:45 AM
3 protein family expansions were discovered in R. undulata. The HSP20 expansion was present in the other fire-associates, but the 2 others were unique to R. undulata! This included GST and ACD enzymes. See below! 🔽
November 14, 2025 at 7:45 AM
Excited to share our newest article, out in BMC Genomics!

We looked at genomes of a bunch of Pezizomycetes without & without associations with fire to figure out how Rhizina undulata survives fire 🔥

R. undulata needs high temperatures for germination = fire is important for its life cycle 🔥
November 14, 2025 at 7:45 AM
Attending the annual #PlantBiologicalsNetworkMeeting in Copenhagen today ✨️
November 11, 2025 at 9:57 AM
The most elegant diagram to explain what the scientific process and the average research project looks like ✨️

So many side tracks that eventually don't make it into the final story, but are an integral part of the process.

From Yanai & Lercher (2025) in Nature Biotech
May 22, 2025 at 7:23 AM
56 RNAseq samples done and ready to be shipped! 🥳🧬🧫🧪🔬
May 12, 2025 at 5:07 PM
Seeing Jennifer Doudna speak this afternoon at the Black Diamond in Copenhagen 🧬🧫🔬

Appropriately dressed in my #CRISPREngineer shirt from @thermofishersci.bsky.social 🧬💁‍♀️
May 2, 2025 at 12:30 PM
10 years ago, I published my first, first-author paper 🤩

In some ways, it feels like just yesterday and I'm still a baby masters student 🥹

In other ways, it feels like this was 100 years ago 👵

Either way, the joy of seeing your work published never goes away ❤️
April 28, 2025 at 5:54 AM
Blood. Sweat. Tears.

Locust & root media.

🧪🔬🧫
🦗🌾
April 25, 2025 at 6:10 PM
🧪🧬 We also provide a schematic and lots of in text details on how you can optimize this protocol for use in your own species! 🧬🧪
April 3, 2025 at 1:08 PM
🧪🧬 Protocol 5 is for Sclerotinia sclerotiorum- the fungus with a host range of more than 400 plant species! 🌾🍀🌿🌳 This protocol uses immature mycelium as a starting point
April 3, 2025 at 1:08 PM
🧪🧬 Protocol 4 is for Ophiostoma novo-ulmi, the Dutch elm disease pathogen 🌲🍄 This protocol uses a DTT-EDTA pre-treatment and uses conidia as the starting material ⚗️
April 3, 2025 at 1:08 PM
🧪🧬 Protocol 3 is for Metarhizium species- fungi that infect and cause death in insects, including various agricultural pests 🦗 The protocol uses mature mycelium as a starting point.
April 3, 2025 at 1:08 PM
🧪🧬 Protocol 2 is for Fusarium circinatum- the notorious pine pathogen that causes devastation in natural and managed forests 🌲 This protocol describes extraction from a mixture of mycelium and conidia
April 3, 2025 at 1:08 PM
🧪🧬 Protocol 1 is for Ceratocystis species and describes the extraction of protoplasts from mycelium, using a density gradient to purify the protoplasts 🍄🌲
April 3, 2025 at 1:08 PM
We present 5 protocols, each optimized in a different fungal genus, for species important in agriculture 🌾 & forestry 🌲

We use an enzyme from the wine industry, #Extralyse, to overcome the reliance on cost-prohibitive lab-grade enzymes whose availability is unreliable 🥲🧪🧬
April 3, 2025 at 1:08 PM
Super excited to share our newest paper, out in
Current protocols!

A collab btwn FABI & UCPH, we put together protoplast extraction protocols for 5 different fungal genera! 🍄

With applications for genome editing & long read seq, we hope this will be super useful! 🧪🧬
April 3, 2025 at 1:08 PM
It's taken a while, but I'm finally trying a #MigraineHat.. 😅

I've been an icepack girlie for a very long time, but this 360° cooling effect is 👌

Not to mention the sound and light dulling effect too 😎

#Migraine #ChronicMigraine
March 22, 2025 at 11:57 AM
And with that, another 4000-odd seeds have been sowed again 🌱💪
March 21, 2025 at 6:47 PM
Wednesday was the first sampling day and... my root and locust media was contaminated by this bacteria left 🧫

But after some tears and a restless night, I know what went wrong and how to fix it 💪

Hopefully with the added experience, setting up Round 2 won't take as long! 🤞
March 21, 2025 at 1:45 PM
This little plate and its tiny bacteria left me heartbroken on Wednesday evening 💔

After months of planning and preparing, a pre-pilot trial and a pilot trial, I started my large RNAseq experiment last week 🥳

If everything went to plan, I'd be done by 28 March. But biology had other plans... 🧬
March 21, 2025 at 1:45 PM
March 11, 2025 at 9:02 PM
And I can finally say that my pilot experiment starts tomorrow 💪🌱🦗

If everything goes according to plan, the full experiment starts week after next!

Let's go! 🤩 #MycoSky #FungiSky #RNASeq
March 6, 2025 at 8:33 PM
The hardest ~35g I've ever worked for 😅🌱

Harvesting roots for this experiment has been a challenge 💪

Turns out that 360g of wet weight barley root = 36g dry weight 😂

But we've got the goods and the pilot experiment is underway!
March 4, 2025 at 8:06 AM
Never gonna get bored of :
1) "Dear Dr Wilson"
2) "... accept your manuscript" 🥳🥳

Happy Monday! 😅
March 3, 2025 at 4:09 PM