Hi Farin, we have had mixed results with transgenic reporters. I would say that using them with ATLAS is “off-label”, but you are welcome to try. We are currently trying to figure out what is causing the variation and hope to be able to publish something on it in the near future.

ATLAS is more specific than previous anterograde tracers like AAV1 because it can trace from genetically determined cells and is strictly transsynaptic. It is not as efficient as rabies (maybe 50%), but also likely causes less toxicity.

Only problem is that it doesn’t work in cells without synaptotagmin 1 (inhibitory neurons, dopaminergic neurons, etc.). Any chance you have developed nanobodies against other synaptotagmins?

Synaptotagmin 1 nanobody is great! Far superior to vamp2 overexpression.

I signed up for research kick on march 19 for $30/month. Now when i sign in it has no memory of me and there doesn’t seem to be a way of stopping the payments. You can’t be messaged, so i need to do this in public unfortunately. Please give me instructions for how to stop my subscription.

He was just being honest- probably a mistake. He also said some very nice things about Barkley. Must be difficult to watch others do what you are no longer able to.

Thanks, Nathalie! Hope you and Emre are keeping warm and enjoying the northern lights.

The problem seems to be that even though it is possible to acquire followers on bluesky, engagement seems to be <10% of what it was on X.

We believe that these tools will work even if they don’t have an impact factor.

Reviewer 3!

Sorry to hear that. It’s incredibly frustrating when innovation is penalized.