13. Lastly, thank you to my other co-authors and all those who provided feedback on the paper!

12. Second, all our lab members who contributed time and energy to methods and experiments, including Sunhye Lee, Yeha Kim, Jennifer Aparicio, Jay Bai, and Linda Cambier.

11. First, our informatics specialists Kevin Stachalek, Mitali Singh, and Bhavana Bhat who did so much work getting our pipeline functional and performing follow up analyses.

10. I have to thank my mentor David Cobrinik for his guidance. I also need to thank the lab members and coauthors for all the teaching and support they have given me over the years.

9. Lastly, we returned to the processes associated with retinoblastoma. and found that cones had increased SYK expression and that inhibition of SYK decreased cone proliferative response to RB1 loss in vitro indicating it plays a role in retinoblastoma initiation.

8. We further identified new features of early maturing rods and cones, including shared CHRNA1 expression and sequential expression of four lncRNAs in L/M cones.

7. The early cone and rod populations contained cells closely related in umap space resembling a possible post-mitotic hybrid state. However, in situ analyses showed early cone precursors with rod gene expression but not vice versa, suggesting this group was likely developing cones not a hybrid.

6. Examining the transition from progenitors to PR clusters under higher resolution identified two small postmitotic populations trajecting toward rods and cones that expressed genes and regulons associated with rods or cones, suggesting fate specification happening directly from the RPCs.

5. Interestingly, our rod and cone precursors co-expressed canonical markers of the other cell type (NRL and THRB). Isoform analyses showed differences between cell types: L/M cones showed use of a truncated NRL mRNA while late rods showed more THRB premature termination events.

4. Our dataset captured clusters of retinal progenitors, early and late rods, and L/M and S cones with some novel cell type-specific genes and ontologies. We further defined these clusters by examining their expression of individual transcription factors and coregulated target genes (regulons).

3. Instead of using current droplet-based end-counting techniques, postdoctoral fellow Sunhye Lee established a process to collect photoreceptor-enriched single cells directly from a FACS machine and sequence full length transcripts.

2. We focused on the maturation of cone photoreceptors, the cells in the human retina which can give rise to retinoblastoma. We aimed to build a clearer picture of photoreceptor fate specification and post-mitotic maturation states through single- cell RNA sequencing of developing human tissues.

That's fantastic! Is that your first time meeting Deborah?

Her whole soul was destroyed and she bore it to the world. What a powerful act no-one should ever have to perform. RIP Emmett

Thanks for the book Rob, it was a ton of fun and I also want so much more info about what's going on already. XD it feels like there could be a dozen books in this series!