I have been thinking about this question too. Part of me was worried that doing this would damage the entire system in a way that will be used against scientists to justify further reductions.

Welcome to the club 😔. You’ve got a big voice. Hopefully you can continue to advocate strongly for science—particularly with your local representatives and community members where changed minds can have a meaningful impact.

I hear you. If this was a well-studied protein with a rock-solid antibody I might consider it. But it’s not. Also, it’s an antiquated double standard to “validate” more specific and quantitative proteomics data with an inferior western blot assay. I would bet the opposite request is far more rare.

Usually it’s one 10cm or 15cm dish of cells. But this is way more than needed, since we only inject ~5% of the sample after IP.

👏 Philly was a great host city. Women-friendly state laws, easy train ride from the airport to the city, and most of all the people of the city were so nice and helpful. 10/10 would go again.

Thanks for the shoutout on our paper. It was a difficult process of methods optimization over several years to get this to work well and provide biologically meaningful results.