Devon Kavanaugh
@dkavanaugh-glyco.bsky.social
Glycobiologist / biochemist
Researcher of all things host:microbe interactions.
I like mucus and volleyball.
Researcher of all things host:microbe interactions.
I like mucus and volleyball.
3) SDS-PAGE prep in laemmli buffer results in a 90 kDa FimA pentamer across several strains.
This implies the presence of SDS/heat sensitive subunits throughout the pili shaft. This backs up previous evidence of the presence of accessory subunits.
Have a read and let me know what you think.
This implies the presence of SDS/heat sensitive subunits throughout the pili shaft. This backs up previous evidence of the presence of accessory subunits.
Have a read and let me know what you think.
February 9, 2025 at 5:46 PM
3) SDS-PAGE prep in laemmli buffer results in a 90 kDa FimA pentamer across several strains.
This implies the presence of SDS/heat sensitive subunits throughout the pili shaft. This backs up previous evidence of the presence of accessory subunits.
Have a read and let me know what you think.
This implies the presence of SDS/heat sensitive subunits throughout the pili shaft. This backs up previous evidence of the presence of accessory subunits.
Have a read and let me know what you think.
🗝️ Key points of the study:
1) Type I pili are composed of 500-3000 FimA subunits, but they're not all the same! --> Different responses to different reducing agents.
2) Dissociated FimA monomers are recognized by the ConA lectin, but not when assembled in polymers.
1) Type I pili are composed of 500-3000 FimA subunits, but they're not all the same! --> Different responses to different reducing agents.
2) Dissociated FimA monomers are recognized by the ConA lectin, but not when assembled in polymers.
February 9, 2025 at 5:46 PM
🗝️ Key points of the study:
1) Type I pili are composed of 500-3000 FimA subunits, but they're not all the same! --> Different responses to different reducing agents.
2) Dissociated FimA monomers are recognized by the ConA lectin, but not when assembled in polymers.
1) Type I pili are composed of 500-3000 FimA subunits, but they're not all the same! --> Different responses to different reducing agents.
2) Dissociated FimA monomers are recognized by the ConA lectin, but not when assembled in polymers.