PS photos are before and after. We all survived - we just couldn't locate everyone in the dramatic minutes before the race 😅

Thanks @diantoniogroup.bsky.social

Thanks - will do!

Grazie!!!

@francolab.bsky.social @pcicuta.bsky.social

@lasergroup.bsky.social @diantoniogroup.bsky.social @oasaleh.bsky.social @rogerrubiosanchez.bsky.social

Thanks @oasaleh.bsky.social, great suggestion!

Thanks @floppleton.bsky.social Andy Stannard did that

Great collaboration with Alexei's team, ‪@diantoniogroup.bsky.social‬ and many others. Thanks @leverhulme.ac.uk ‪‪@royalsociety.org‬ @erc.europa.eu
‪@imperialchemistry.bsky.social‬ @ceb.cam.ac.uk

We believe that this simple but modular condensate design strategy, based on nanostars and linkers, is really powerful and flexible. Hopefully others will find it useful too. Thanks to @erc.europa.eu @royalsociety.org @ceb.cam.ac.uk for funding and support! n/n

Finally, we explore the effect on phase behaviour of changing annealing protocols, demonstrating that multicomponent DNA condensates equilibrate veeeeeeeeeeery slowly. Something to bear in mind if you plan to work with similar systems 5/n

Simulations by Dino Osmanovic @francolab.bsky.social, based on Flory-Huggins, map well onto the experimental trends and allowed us to establish a link between the experimental order parameter and the F-H interaction parameter. Experimental (top) and simulated (bottom) snapshots below 4/n

We can control phase behaviour by changing linker and nanostars concentrations, without having to re-design nanostructures. This is rather convenient, and allowed us to map a large phase diagram and identify an order parameter controlling the transition between 1- and 2-phase condensates 3/n

Condensates assemble from two populations of tetravalent DNA nanostars, and three types of divalent linkers that mediate nanostar-nanostar interactions, either within the same nanostar population or across populations 2/n

Grazie Otti!

Thanks @ceb.cam.ac.uk @erc.europa.eu @royalsociety.org and BBSRC n/n

The MLO platform is robust, inducible and modular and many design modifications can be applied to the nanostars to capture different proteins or change MLO properties. Hopefully you’ll find this useful, and we look forward to your feedback! 9/n