I'm planning on running an olink pilot study so I'll need to get up to speed in this. I see the Coons Lab did a comparison

www.biorxiv.org/content/10.1...

Am I being silly, but I didn't think somalogic used antibodies. I thought they used protein binding DNA?

Also consider double digestion. I'm currently running where a specific C is being targeted. The only way we could detect it was digesting with Trypsin and AspN together

The underside is usually pretty boring; designed to disappear into the sky; maybe painted with sky grey etc and that's all you would see hung up.

I have started to try and give them away to people. If you are ever in Cambridge, UK I can hook you up 😉

And more competition can only help in driving down costs

I kind of used Bens blog as my 'bot' to be honest. Its curated and i really just don't have the time between work, two young kids etc to read as much as I would like

They were also offering a Lumos at deep discount as well

Thanks. Looks great by the way 👏

I have heard it this, but never tried it. Do you dilute the putty?

They were from an older kit that I found at a modelling show.

It's not daft, it's asked the same question (I am not a FAIMS expert). Their argument was that choosing only CV is too selective and will not produce results representative of the whole sample. To ensure we are not filtering out certain peptides, we need at least 2 CV's.

I recently completed one with folded Wings...... Is is very fragile

Thanks for the link. Think it will be a really good topic for a lit review session

Linkedin is also connected with my employer (my manager, people I manage, directors ect). I'm definitely so not feel as open for discussion on it. I find it very hard on Twitter to find things in interested in lately so hoping here works.

We have an Exploris. Instrument time is too limited to run on multiple injections. I want to see if we can run with FAIMS for all our workflows as taking it on/off is inefficient, but the team have said DIA requires two CV voltages

We are running out with some PRM stuff and we still do TMT. Currently not using it for DIA as we use DIAnn for analysis. My experience had been mixed.