Denis Wirtz
@deniswirtz.bsky.social
VP for Research, T.H. Smoot Professor
Depts Chemical and Biomolecular Engn, Pathology, Oncology, INBT
Johns Hopkins University
3D multi-omic, CAR T therapy, cell migration and mechanics
Lab: https://wirtzlab.johnshopkins.edu
Depts Chemical and Biomolecular Engn, Pathology, Oncology, INBT
Johns Hopkins University
3D multi-omic, CAR T therapy, cell migration and mechanics
Lab: https://wirtzlab.johnshopkins.edu
New paper out with colleagues at the Buck Institute.
Senescent cell heterogeneity and responses to senolytic treatment are related to cell cycle status during senescence induction
More here: www.aging-us.com/article/206299
Senescent cell heterogeneity and responses to senolytic treatment are related to cell cycle status during senescence induction
More here: www.aging-us.com/article/206299
November 22, 2025 at 3:05 PM
New paper out with colleagues at the Buck Institute.
Senescent cell heterogeneity and responses to senolytic treatment are related to cell cycle status during senescence induction
More here: www.aging-us.com/article/206299
Senescent cell heterogeneity and responses to senolytic treatment are related to cell cycle status during senescence induction
More here: www.aging-us.com/article/206299
Still my favorite 3D reconstruction of a pancreatic tissue.
Using the CODA workflow, we mapped a billion individual cells in 3D.
Red: pre-cancer lesions
Navy blue: ducts
Light blue: islets of Langerhans
Purple: acini
Green: blood vessels
Yellow: fat
More here: www.nature.com/articles/s41...
Using the CODA workflow, we mapped a billion individual cells in 3D.
Red: pre-cancer lesions
Navy blue: ducts
Light blue: islets of Langerhans
Purple: acini
Green: blood vessels
Yellow: fat
More here: www.nature.com/articles/s41...
November 20, 2025 at 6:34 PM
Still my favorite 3D reconstruction of a pancreatic tissue.
Using the CODA workflow, we mapped a billion individual cells in 3D.
Red: pre-cancer lesions
Navy blue: ducts
Light blue: islets of Langerhans
Purple: acini
Green: blood vessels
Yellow: fat
More here: www.nature.com/articles/s41...
Using the CODA workflow, we mapped a billion individual cells in 3D.
Red: pre-cancer lesions
Navy blue: ducts
Light blue: islets of Langerhans
Purple: acini
Green: blood vessels
Yellow: fat
More here: www.nature.com/articles/s41...
Reposted by Denis Wirtz
“I’m currently involved in a study where we’re using these technologies to study pancreatic cancers,” says @jhu-bdps.bsky.social @mikeschatz.bsky.social. We’re finding things that are only possible because of this new genome and these new technologies.”
Putting our pieces together
For more than half a century, Johns Hopkins has been a leader in converting federal support into tangible benefits for the American people—such as correcting maps of the human genome.
www.cs.jhu.edu
November 20, 2025 at 2:27 PM
“I’m currently involved in a study where we’re using these technologies to study pancreatic cancers,” says @jhu-bdps.bsky.social @mikeschatz.bsky.social. We’re finding things that are only possible because of this new genome and these new technologies.”
Color-coded CODA maps of fallopian tubes from premenopausal (left) and postmenopausal women.
Magenta: secretory epithelial cells,
Green: ciliated epithelial cells.
More here: www.biorxiv.org/content/10.1...
Magenta: secretory epithelial cells,
Green: ciliated epithelial cells.
More here: www.biorxiv.org/content/10.1...
November 16, 2025 at 3:46 PM
Color-coded CODA maps of fallopian tubes from premenopausal (left) and postmenopausal women.
Magenta: secretory epithelial cells,
Green: ciliated epithelial cells.
More here: www.biorxiv.org/content/10.1...
Magenta: secretory epithelial cells,
Green: ciliated epithelial cells.
More here: www.biorxiv.org/content/10.1...
Another pic of colleague Prof Earle Havens showing us a gigantic map inserted into the monumental Piranesi’s “Views of Rome” that appeared in the 18th century.
What a privilege to have such a great colleague
What a privilege to have such a great colleague
November 12, 2025 at 2:10 PM
Another pic of colleague Prof Earle Havens showing us a gigantic map inserted into the monumental Piranesi’s “Views of Rome” that appeared in the 18th century.
What a privilege to have such a great colleague
What a privilege to have such a great colleague
Colleague Prof. Earle Havens is showing a group of Hopkins faculty an original Audubon’s Birds of America, elephant folio at the Evergreen house in Baltimore
November 11, 2025 at 12:55 PM
Colleague Prof. Earle Havens is showing a group of Hopkins faculty an original Audubon’s Birds of America, elephant folio at the Evergreen house in Baltimore
We have written two reviews providing a practical guide and fundamentals of cell migration:
(1) How to select an optimal cell migration assay,
(2) How to analyze cell migration data.
We also added a perspective on AI methods of analysis.
Coming out soon in Nature Methods.
(1) How to select an optimal cell migration assay,
(2) How to analyze cell migration data.
We also added a perspective on AI methods of analysis.
Coming out soon in Nature Methods.
November 8, 2025 at 6:17 PM
We have written two reviews providing a practical guide and fundamentals of cell migration:
(1) How to select an optimal cell migration assay,
(2) How to analyze cell migration data.
We also added a perspective on AI methods of analysis.
Coming out soon in Nature Methods.
(1) How to select an optimal cell migration assay,
(2) How to analyze cell migration data.
We also added a perspective on AI methods of analysis.
Coming out soon in Nature Methods.
Our recent observations are distinct from conventional active matter. Self-propelled colloidal particles neither secrete inhibitory molecules, nor actively modulate adhesion molecules, which are two key ingredients leading to our observed patterned aggregates.
Here: www.nature.com/articles/s41...
Here: www.nature.com/articles/s41...
November 8, 2025 at 1:54 PM
Our recent observations are distinct from conventional active matter. Self-propelled colloidal particles neither secrete inhibitory molecules, nor actively modulate adhesion molecules, which are two key ingredients leading to our observed patterned aggregates.
Here: www.nature.com/articles/s41...
Here: www.nature.com/articles/s41...
We have observed a beautifully strange phenomenon of phase separation by human monocytes placed on soft matrix, which do it for survival.
We model it by incorporating the Cahn–Hilliard equation with a Turing mechanism of local activation/global inhibition
More here: www.nature.com/articles/s41...
We model it by incorporating the Cahn–Hilliard equation with a Turing mechanism of local activation/global inhibition
More here: www.nature.com/articles/s41...
Mechano-induced patterned domain formation by monocytes - Nature Materials
Human primary monocytes reversibly phase separate into regular, multicellular, multilayered domains on soft matrices with physiological stiffness due to local activation and global inhibition processe...
www.nature.com
November 5, 2025 at 12:41 PM
We have observed a beautifully strange phenomenon of phase separation by human monocytes placed on soft matrix, which do it for survival.
We model it by incorporating the Cahn–Hilliard equation with a Turing mechanism of local activation/global inhibition
More here: www.nature.com/articles/s41...
We model it by incorporating the Cahn–Hilliard equation with a Turing mechanism of local activation/global inhibition
More here: www.nature.com/articles/s41...
Immune hot spots in human tissues mapped with our CODA workflow.
When zoomed in, it looks like abstract art.
When zoomed in, it looks like abstract art.
October 31, 2025 at 5:53 PM
Immune hot spots in human tissues mapped with our CODA workflow.
When zoomed in, it looks like abstract art.
When zoomed in, it looks like abstract art.
Good night and good luck
October 30, 2025 at 10:41 PM
Good night and good luck
Reposted by Denis Wirtz
I wrote an op-ed on the world-class STEM research ecosystem in the United States, and how this ecosystem is now under attack on multiple fronts by the current administration: newsletter.ofthebrave.org/p/im-an-awar...
I’m an award-winning mathematician. Trump just cut my funding.
The “Mozart of Math” tried to stay out of politics. Then it came for his research.
newsletter.ofthebrave.org
August 18, 2025 at 3:45 PM
I wrote an op-ed on the world-class STEM research ecosystem in the United States, and how this ecosystem is now under attack on multiple fronts by the current administration: newsletter.ofthebrave.org/p/im-an-awar...
Reposted by Denis Wirtz
"The scientific hegemony the USA had for decades has gone...the percentage of papers with a Chinese author [i]n 2025 is 23%, the USA 11%...transition to open access is only possible if Chinese researchers change their publishing habits" newsletter.journalology.com/p/the-rise-o...
The rise of China and the fall of open access
The theme of Open Access Week is "Who owns our knowledge?". The answer is increasingly likely to be "China".
newsletter.journalology.com
October 25, 2025 at 10:33 PM
"The scientific hegemony the USA had for decades has gone...the percentage of papers with a Chinese author [i]n 2025 is 23%, the USA 11%...transition to open access is only possible if Chinese researchers change their publishing habits" newsletter.journalology.com/p/the-rise-o...
Colleague Prof. Max Konig and Johns Hopkins colleagues show that anti-9G4 precision cellular therapies provide a strategy to selectively target pathogenic B cells in lupus, while minimizing risks of infection and cytokine-related toxicities.
Read more here: www.biorxiv.org/content/10.1...
Read more here: www.biorxiv.org/content/10.1...
October 25, 2025 at 7:28 PM
Colleague Prof. Max Konig and Johns Hopkins colleagues show that anti-9G4 precision cellular therapies provide a strategy to selectively target pathogenic B cells in lupus, while minimizing risks of infection and cytokine-related toxicities.
Read more here: www.biorxiv.org/content/10.1...
Read more here: www.biorxiv.org/content/10.1...
Fall colors at Hopkins
October 24, 2025 at 11:53 AM
Fall colors at Hopkins
Health economist extraordinaire Dan Polski can take a selfie…
This is my team; their passion is to support Hopkins researchers
This is my team; their passion is to support Hopkins researchers
October 24, 2025 at 11:35 AM
Health economist extraordinaire Dan Polski can take a selfie…
This is my team; their passion is to support Hopkins researchers
This is my team; their passion is to support Hopkins researchers
We are celebrating the 150th anniversary of Johns Hopkins University this year. We’re using the wonderful Peabody Library to host our great faculty for a great evening of celebrations
October 23, 2025 at 9:54 PM
We are celebrating the 150th anniversary of Johns Hopkins University this year. We’re using the wonderful Peabody Library to host our great faculty for a great evening of celebrations
It’s that time of the year again when we celebrate the amazing achievements and discoveries made by Johns Hopkins faculty.
We take over the Peabody Library so that awardees of our internal grants get a chance to talk to one another and forge new collaborations
We take over the Peabody Library so that awardees of our internal grants get a chance to talk to one another and forge new collaborations
October 23, 2025 at 9:48 PM
It’s that time of the year again when we celebrate the amazing achievements and discoveries made by Johns Hopkins faculty.
We take over the Peabody Library so that awardees of our internal grants get a chance to talk to one another and forge new collaborations
We take over the Peabody Library so that awardees of our internal grants get a chance to talk to one another and forge new collaborations
Latrobe Hall at Johns Hopkins University
October 23, 2025 at 1:30 PM
Latrobe Hall at Johns Hopkins University
High-throughput screening of drugs that affect cell proliferation is easily done using a plate reader.
No such luck for cell migration. So, we developed an assay for label-free deep learning-based automated cell tracking.
Read more about our assay here: www.biorxiv.org/content/10.1...
No such luck for cell migration. So, we developed an assay for label-free deep learning-based automated cell tracking.
Read more about our assay here: www.biorxiv.org/content/10.1...
October 22, 2025 at 12:25 AM
High-throughput screening of drugs that affect cell proliferation is easily done using a plate reader.
No such luck for cell migration. So, we developed an assay for label-free deep learning-based automated cell tracking.
Read more about our assay here: www.biorxiv.org/content/10.1...
No such luck for cell migration. So, we developed an assay for label-free deep learning-based automated cell tracking.
Read more about our assay here: www.biorxiv.org/content/10.1...
L’Empire des Lumières
October 21, 2025 at 10:18 PM
L’Empire des Lumières
Homewood campus is glorious today
Johns Hopkins
Johns Hopkins
October 21, 2025 at 3:15 PM
Homewood campus is glorious today
Johns Hopkins
Johns Hopkins
We use CODA to map human endometrial tissue at single-cell resolution, a map that then serves as a blueprint to design a multi-compartment multicellular assembloid of the endometrium.
Read about our approach to organoids/assembloids here: www.biorxiv.org/content/10.1...
Read about our approach to organoids/assembloids here: www.biorxiv.org/content/10.1...
October 18, 2025 at 3:41 PM
We use CODA to map human endometrial tissue at single-cell resolution, a map that then serves as a blueprint to design a multi-compartment multicellular assembloid of the endometrium.
Read about our approach to organoids/assembloids here: www.biorxiv.org/content/10.1...
Read about our approach to organoids/assembloids here: www.biorxiv.org/content/10.1...
Pretty Fall colors at Homewood
Johns Hopkins university
Johns Hopkins university
October 17, 2025 at 1:57 PM
Pretty Fall colors at Homewood
Johns Hopkins university
Johns Hopkins university
Prof. Jasmina Wiemann has been selected for a prestigious Packard fellowship. This the first recognition of a paleobiologist by the Foundation!
That is 8 Packard Fellows in 6 years for Johns Hopkins University.
Prof. Wiemann's website is the stuff of movies: www.jasminawiemann.com
That is 8 Packard Fellows in 6 years for Johns Hopkins University.
Prof. Wiemann's website is the stuff of movies: www.jasminawiemann.com
October 15, 2025 at 1:54 PM
Prof. Jasmina Wiemann has been selected for a prestigious Packard fellowship. This the first recognition of a paleobiologist by the Foundation!
That is 8 Packard Fellows in 6 years for Johns Hopkins University.
Prof. Wiemann's website is the stuff of movies: www.jasminawiemann.com
That is 8 Packard Fellows in 6 years for Johns Hopkins University.
Prof. Wiemann's website is the stuff of movies: www.jasminawiemann.com